Characteristics of the classical complement pathway (CCP) of rainbow trout Salmo gairdneti was examined using sheep red blood cells sensitized with porgy Pagrms major antibody which is compatible with rainbow trout complement. The rainbow trout CCP showed the highest activity when the reaction was performed at 20-25℃ and around pH 7.5, and the hemolysis reached a plateau in about 120 min. It was found that a considerable hemolysis which is attributable to the alternative complement pathway (ACP) of rainbow trout took place in the presence of 10 mM Mg^2+. The hemolysis caused by the ACP, however, was less than 10% of the total hemolysis when the hemolytic reaction was performed in the presence of 1 mM Ca^<2+> and 1 mM Mg^<2+>.
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