Transfection and Transformation Systems for Pock-Forming and Thiostrepton-Producing Streptomyces azureus - Collections

＜departmental bulletin paper＞
Transfection and Transformation Systems for Pock-Forming and Thiostrepton-Producing Streptomyces azureus

Creator	Author PID L002941 Creator Name Ogata, Seiya 緒方, 靖哉 Affiliation Affiliation Name Laboratory of Applied Microbiology, Faculty of Agriculture, Kyushu University 九州大学農学部農芸化学科発酵学講座
	Creator Name Koyama-Miyoshi, Yuko Affiliation Affiliation Name Laboratory of Applied Microbiology, Faculty of Agriculture, Kyushu University
	Creator Name Hayashida, Shinsaku 林田, 晋策 Affiliation Affiliation Name Laboratory of Applied Microbiology, Faculty of Agriculture, Kyushu University 九州大学農学部農芸化学科発酵学講座
Language	English
Publisher	Kyushu University
Publisher	九州大学農学部
Date	1985-06
Source Title	Journal of the Faculty of Agriculture, Kyushu University
Vol	29
Issue	4
First Page	179
Last Page	188
Publication Type	Version of Record
Access Rights	open access
Crossref DOI	https://doi.org/10.5109/23804
Related DOI	九州大学農学部紀要 \|\| 29(4) \|\| p179-188
	Journal of the Faculty of Agriculture, Kyushu University \|\| 29(4) \|\| p179-188
	http://www.agr.kyushu-u.ac.jp/
Related URI	九州大学農学部紀要 \|\| 29(4) \|\| p179-188
	Journal of the Faculty of Agriculture, Kyushu University \|\| 29(4) \|\| p179-188
	http://www.agr.kyushu-u.ac.jp/
Relation	九州大学農学部紀要 \|\| 29(4) \|\| p179-188
	Journal of the Faculty of Agriculture, Kyushu University \|\| 29(4) \|\| p179-188
	http://www.agr.kyushu-u.ac.jp/

File	FileType	Size	Views	Description
p179	pdf	598 KB	477

PISSN	0023-6152
NCID	AA00697606
Record ID	23804
Peer-Reviewed	Unrefereed
Subject Terms	Streptomyces azureus
	Pock-Forming
	Thiostrepton-Producing
ISSN	0023-6152
NCID	AA00697606
Notes	To establish a procedure for high frequency transfection and transformation of Streptomyces azureus, at first, the condition for the protoplast formation and regeneration of this strain were studied. The effective formation and regeneration of protoplasts of S. azureus were obtained by using mycelia grown in the presence of 0.4 % glycine. The effective transfection of S. atureus protoplasts with temperate phage SAt 1 DNA was obtained at 20 % (W/V) PEG 2000. The frequency of transfection under the optimal condition was about 1 per 5×10^6 DNA molecule (equal to 5×10^3 transfectants per tig DNA) and 1 per 5.3×10^3 regenerated protoplasts. The DNA transfection of a virulent phage SAvl was also succeeded in the protoplasts of S. azureus. The transformation of pock-cured strain AF-6 of S. azureus with pock-inducing plasmid DNA occurred under the same condition as transfection of phage SAtl DNA.
Created Date	2012.08.01
Modified Date	2022.11.11