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＜departmental bulletin paper＞
Effects of Exogenous Phospholipase A_2 and C on Phospholipids in Whole Cells of Escherichia coli JE1011 and Its NS Mutants

Creator
Creator Name
Tatsuguchi, Kazue
竜口, 和恵
Affiliation
Affiliation Name
Food Hygienic Chemistry, Faculty of Agriculture, Kyushu University
九州大学農学部食品衛生化学講座
Creator Name
Kai, Tatsuo
甲斐, 達男
Affiliation
Affiliation Name
Food Hygienic Chemistry, Faculty of Agriculture, Kyushu University
九州大学農学部食品衛生化学講座
Creator Name
Tsuruta, Michiko
Affiliation
Affiliation Name
Food Hygienic Chemistry, Faculty of Agriculture, Kyushu University
Author PID
L000569
Creator Name
Watanabe, Tadao
渡辺, 忠雄
Affiliation
Affiliation Name
Food Hygienic Chemistry, Faculty of Agriculture, Kyushu University
九州大学農学部食品衛生化学講座
Language
English
Publisher
Kyushu University
九州大学農学部
Date
1982-07
Source Title
Journal of the Faculty of Agriculture, Kyushu University
Vol
26
Issue
4
First Page
193
Last Page
203
Publication Type
Version of Record
Access Rights
open access
Crossref DOI
https://doi.org/10.5109/23749
Related DOI
九州大学農学部紀要 || 26(4) || p193-203
Journal of the Faculty of Agriculture, Kyushu University || 26(4) || p193-203
http://www.agr.kyushu-u.ac.jp/
Related URI
九州大学農学部紀要 || 26(4) || p193-203
Journal of the Faculty of Agriculture, Kyushu University || 26(4) || p193-203
http://www.agr.kyushu-u.ac.jp/
Relation
九州大学農学部紀要 || 26(4) || p193-203
Journal of the Faculty of Agriculture, Kyushu University || 26(4) || p193-203
http://www.agr.kyushu-u.ac.jp/

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Details

PISSN
0023-6152
NCID
AA00697606
Record ID
23749
Peer-Reviewed
Unrefereed
Subject Terms
Ethylenediaminetetraacetate (EDTA)
NS mutants
Escherichia coli JE 1011
ISSN
0023-6152
NCID
AA00697606
Notes
Whole cells of Escherirhia coli JE 1011 and its novobiocin-supersensitive (NS) mutants were treated with exogenous phospho!ipase A_2 and C. The phospholipids in whole cells of JE 1011 were scarcely hydrolyzed by the phospholipases, whereas those of NS mutants including NS6 with complete lipopolysaccharide (LPS) were extensively hydrolyzed. The accessibility of phospholipids in whole cells to exogenous phospholipases bore no relation to the composition of phospholipids in these strains. When the cells of JE 1011 were frozen-thawed or treated with ethylenediaminetetraacetate (EDTA), the phospholipids were hydrolyzed by the phospholipases to the extent similar to those of NS mutants. Parent strain JE 1011 was resistant to hydrophobic substances such as tripropyl- and tributyl-tin. gentian violet and esters of p-hydroxybenzoic acid, however, the strain became sensitive to these compounds by EDTA-treatment. On the other hand, all of the NS mutants were sensitive to them. From these results, it was suggested that the accessibility of phospholipids in whole cells to the exogenous phospholipases related to the permeability of hydrophobic substances.
Created Date 2012.07.31
Modified Date 2022.11.11

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