Transformation of Pseudomonas aeruginosa with Chromosomal DNA into Bacillus subtilis - Collections

＜departmental bulletin paper＞
Transformation of Pseudomonas aeruginosa with Chromosomal DNA into Bacillus subtilis

Creator	Author PID K001564 Creator Name Hara, Toshio 原, 敏夫 Affiliation Affiliation Name Laboratory of Microbial Technology, Faculty of Agriculture, Kyushu University 九州大学農学部食糧工学科微生物工学講座
Creator	Creator Name Ueda, Seinosuke 上田, 誠之助 Affiliation Affiliation Name Laboratory of Microbial Technology, Faculty of Agriculture, Kyushu University 九州大学農学部食糧工学科微生物工学講座
Language	English
Publisher	Kyushu University
Publisher	九州大学農学部
Date	1982-07
Source Title	Journal of the Faculty of Agriculture, Kyushu University
Vol	26
Issue	4
First Page	151
Last Page	157
Publication Type	Version of Record
Access Rights	open access
Crossref DOI	https://doi.org/10.5109/23746
Related DOI	九州大学農学部紀要 \|\| 26(4) \|\| p151-157
	Journal of the Faculty of Agriculture, Kyushu University \|\| 26(4) \|\| p151-157
	http://www.agr.kyushu-u.ac.jp/
Related URI	九州大学農学部紀要 \|\| 26(4) \|\| p151-157
	Journal of the Faculty of Agriculture, Kyushu University \|\| 26(4) \|\| p151-157
	http://www.agr.kyushu-u.ac.jp/
Relation	九州大学農学部紀要 \|\| 26(4) \|\| p151-157
	Journal of the Faculty of Agriculture, Kyushu University \|\| 26(4) \|\| p151-157
	http://www.agr.kyushu-u.ac.jp/

File	FileType	Size	Views	Description
p151	pdf	145 KB	350

PISSN	0023-6152
NCID	AA00697606
Record ID	23746
Peer-Reviewed	Unrefereed
Subject Terms	Pseudomonas aeruginosa
	Bacillus subtilis
	Chromosomal DNA
ISSN	0023-6152
NCID	AA00697606
Notes	Successful transformation of gram-negative and gram-positive bacteria was achieved. Genetic transformation of Pseudomonas aeruginosa KYU-1 to Bacillus subtilis Marburg 168 was performed with linear chromosamal DNA, provided the cells were treated with cold calcium chloride and a heat pulse. The molecular weight of transforming DNA of the donor was markedly dependent on the transformation frequency. At 6.2x10^6 daltons, transformation frequency with chromosomal DNA of P. aeruginosa into B. subtilis was obtained at 2.0x10-5. The divalent ions such as Ca^<2+> and Mg^<2+> enhanced transformation frequency.
Created Date	2012.07.31
Modified Date	2022.11.11