Antioxidant activities were estimated with methanol extracts of callus cultures derived from cambial parts of Pinus thunbergii Parl., Pinus pentaphylla Mayr., Pinus koraiensis Sieb. et Zucc. and Cryptomeria japonica D. Don. The extract from P. koraiensis callus cells exhibited the most intensive activity comparable with that of α-tochopherol, a typical natural antioxidant, when assayed by the linoleic acid method ant the ghost method. The antioxidant activity of hydrophilic fractions of the extract is likely to be accounted for by their ability of the complex formation with iron and other heavy metals which catalyze lipid autoxidation. The radical scavenging mechanism may contribute, at least in part, to the antioxidant activity shown by the extracts from P. koraiensis callus. However, they did not show the SOD mimic activity.