Genetic analyses were carried out to determine the loci of resistance to green rice leafhopper (GRLH) (Nephotettix cincticeps UHLER). Near isogenic lines with GRLH resistance genes were developed by backcrossing four times using GRLH susceptible cultivar, Kinmaze as recurrent parents and DV 85 as a donor parent. Genetic segregation ratios in the backcrossed F_2 populations indicated two dominant complementary genes govern GRLH resistance of DV 85. RFLP analysis revealed that one of the resistance genes was tightly linked with RFLP marker, XNpb144 on chromosome 3, in both of the two backcrossed F_2 populations, and that the other resistant gene was linked with RFLP markers, XNpb181 and G1465 on chromosome 11 in one of the backcrossed F_2 populations. Among the two RFLP markers on chromosome 11, G1465 was most tightly linked to the resistance gene.