To clarify whether DHA-enriched or iodine egg has anti-allergic effect or not, Sprague-Dawley rat were fed with egg yolk powder of these health oriented eggs at the 10% level and the effects on chemical mediator releasing activity of peritoneal exudate cells. LTB_4 releasing activity of the cells was lowest in the group fed casein and soybean oil, and anti-allergic effect was not observed in the group fed egg yolk powders. In histamine release, its accumulation in the cells was significantly inhibited in normal egg group, while an increasing tendency of the histamine level was observed in DHA enriched and iodine egg groups. In amino acid analysis of liver phospholipids, significant decrease in the proportion of arachidonic acid was observed only in the DHAenriched egg group in phosphatidylcholine. On the contrary, the proportion was higher in all egg yolk powder fed groups than in casein and soybean oil fed group. These results indicate that feeding of egg yolk of DHA-enriched or iodine egg at the 10% level can not induce the decrease of LTB_4 releasing activity, but that feeding of soybean oil containing α-linolenic acid can do.