九州大学農学部造林学講座
Laboratory of Silviculture, Faculty of Agriculture, Kyushu University | Laboratory of Silviculture, Faculty of Agriculture, Kyushu University
In the polyploidization of chromosome by colchicine treatment on seedlings, there was various difficult points. Namely, the ratio of the polyploidization is generally very low and the inducement of polyploid is localized into only a few joints. And so, we tested the use of tissue culture technique to overcome all difficulties in relation to induce of polyploid. The tetraploid plantlets of Niseakasia (Robinia Pseudoacacia L.) were induced effectively by culture of callus originated from seedlings treated with colchicine.