S.cerevisiae 1200 and S.cerevisiae wy2 were isolated as ethanol-tolerant yeast. Aerobically grown cells from spores enhanced ethanol tolerance. S. cerevisiae wy2 showed a novel type of life cycle, referred to as delayed homothallism. Diploidization was extraordinally imduced by the heat treatment(55℃, 10min) during spore germination. The obtained diploid cells with ability to mate were homozygous for mating types and auxotrophic markers. Thus the diploidization was concluded to be endomitotic events in germinating heated spore. A variety of polyploid cells including octaploid cells were constructed using a series of polyploid mater cells.
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