Maternal Transcriptome Analysis of Medaka (Oryzias latipes) Embryos by Use of mRNA–seq - Collections | Kyushu University Library

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＜departmental bulletin paper＞
Maternal Transcriptome Analysis of Medaka (Oryzias latipes) Embryos by Use of mRNA–seq

Creator	Creator Name TAKAI, Yuki 高井, 優生タカイ, ユウキ Affiliation Affiliation Name Laboratory of Marine Environmental Science, Faculty of Agriculture, Kyushu University
	Creator Name CHEN, Kun Affiliation Affiliation Name Laboratory of Marine Environmental Science, Faculty of Agriculture, Kyushu University
	Creator Name YOSHITAKE, Shuhei Affiliation Affiliation Name Laboratory of Marine Environmental Science, Faculty of Agriculture, Kyushu University
	Author PID K006376 Creator Name OGINO, Yukiko 荻野, 由紀子オギノ, ユキコ Affiliation Affiliation Name Attached Promotive Centre for International Education and Research of Agriculture, Faculty of Agriculture, Kyushu University
	Author PID K000715 Creator Name TASHIRO, Kosuke 田代, 康介タシロ, コウスケ Affiliation Affiliation Name Laboratory of Marine Environmental Science, Faculty of Agriculture, Kyushu University
	Author PID K001666 Creator Name SHIMASAKI, Yohei 島崎, 洋平シマサキ, ヨウヘイ Affiliation Affiliation Name Laboratory of Marine Environmental Science, Faculty of Agriculture, Kyushu University 九州大学農学研究院水産生物環境研究室
	Author PID K001575 Creator Name OSHIMA, Yuji 大嶋, 雄治オオシマ, ユウジ Affiliation Affiliation Name Laboratory of Marine Environmental Science, Faculty of Agriculture, Kyushu University 九州大学農学研究院水産生物環境研究室
Language	English
Publisher	Faculty of Agriculture, Kyushu University
Publisher	九州大学大学院農学研究院
Date	2019-09-02
Source Title	Journal of the Faculty of Agriculture, Kyushu University
Vol	64
Issue	2
First Page	275
Last Page	280
Publication Type	Version of Record
Access Rights	open access
Crossref DOI	https://doi.org/10.5109/2340933

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PISSN	0023-6152
NCID	AA00697606
Record ID	2340933
Subject Terms	mRNA–Seq
	maternal transcript
	CYP26A1
	NANOG
Notes	In this study, we investigated the transfer of maternal transcripts to embryos during the early stages of development in medaka (Oryzias latipes). Using mRNA–Seq, we analyzed gene expression levels in medaka embryos, focusing on maternal transcripts during embryonic development from immediately after fertilization to the 7th day post–fertilization. We found that 1,560 transcripts were detected only in the early development stage and that the levels of these transcripts decreased as development progressed. MKRN4, a known medaka maternal transcript, was among the detected transcripts, as were CYP26A1 and NANOG. Our results show that CYP26A1 and NANOG are maternally transported from female fish and play an important role in embryonic development. In this study, we confirmed that using an mRNA–Seq time–course following embryonic development can be used to investigate maternal transcript transfer to embryos.
Funding Information	Funder Name Japan Society for the Promotion of Science 日本学術振興会 Award Number 18H02280 Award Title 魚類リポカリンタンパク質―性分化機構を中心とした機能の解明
Created Date	2019.09.11
Modified Date	2023.11.15