Amperometric enzyme electrode for glucose based on collagen membrane was developed. The glucose oxidase was co-crosslinked with reconstructed collagen using the bifunctional agent, glutaraldehyde. The base electrode used was glassy carbon electrode. Membranes containing 20 units/cm^2 glucose oxidase and 0.05 mg/cm^2 glutaraldehyde gave the most preferable membrane characteristics. The optimum pH and the optimum temperature of the immobilized enzyme membrane was pH 6.6 and 35~40℃, respectively. The enzyme electrode gave the highest response and the best linearity at the potential of +0.9~+1.0 V (vs. Pt). The response time of the enzyme electrode was less than 1 min if a steady-state method was used. The response was proportional to glucose concentration in the 40 μM ~ 1 mM range. The recovery test gave 102 % in average. The enzyme electrode was stable for over a half-year. This electrode is very favorable for glucose assays in food samples.