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＜departmental bulletin paper＞
Gas Chromatographic Analysis of Neutral Steroids in Carp Urine

Creator
Author PID
K001531
Creator Name
矢野, 友紀
Yano, Tomoki
Affiliation
Affiliation Name
九州大学農学部水産化学第一教室
Laboratory of Fisheries Chemistry, Faculty of Agriculture, Kyushu University
Creator Name
石尾, 真弥
Ishio, Shinya
Affiliation
Affiliation Name
九州大学農学部水産化学第一教室
Laboratory of Fisheries Chemistry, Faculty of Agriculture, Kyushu University
Language
Japanese
Publisher
九州大學農學部
Faculty of Agriculture, Kyushu University
Date
1978-09
Source Title
Science bulletin of the Faculty of Agriculture, Kyushu University
Vol
33
Issue
1
First Page
33
Last Page
41
Publication Type
Version of Record
Access Rights
open access
JaLC DOI
https://doi.org/10.15017/23266
Related DOI
九州大學農學部學藝雜誌 || 33(1) || p33-41
Science bulletin of the Faculty of Agriculture, Kyushu University || 33(1) || p33-41
http://www.agr.kyushu-u.ac.jp/
Related URI
九州大學農學部學藝雜誌 || 33(1) || p33-41
Science bulletin of the Faculty of Agriculture, Kyushu University || 33(1) || p33-41
http://www.agr.kyushu-u.ac.jp/
Relation
九州大學農學部學藝雜誌 || 33(1) || p33-41
Science bulletin of the Faculty of Agriculture, Kyushu University || 33(1) || p33-41
http://www.agr.kyushu-u.ac.jp/

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Details

Record ID
23266
Peer-Reviewed
Unrefereed
ISSN
0368-6264
NCID
AN0005519X
Notes
Steroid metabolites in carp (Cyprinus carpio) urine were hydrolyzed with β-glucuronidase and by solvolysis (ethylacetate-1 N H_2SO_4) . The liberated steroids were extracted with ethylacetate, converted to methoxime-trimethylsilyl derivatives, and subjected to gas chromatographic (GC) analysis. The GC separations were carried out on 1 % OV-1 and 2 % QF-1 columns, and the retention data (MU values) of carp urinary steroids ( I -XIII) were compared with those of reference steroids identically treated with the above procedures (Table 1). The structures of the compounds I -X were also confirmed by GC-Mass spectrometry. The results showed the presence of androsterone, etiocholanolone, DHEA, androstenediol, pregnanediol, pregnanetriol, cholesterol, THE, THF, cortolone, β-cortolone, cortol and β-cortol in the urine of carp.
コイ尿中のステロイド代謝産物をβ-グルクロニダーゼとソルボリシス(酢酸エチルーINH_2SO_4)によつて分解し,得られた遊離ステロイドを酢酸エチルで抽出,溶剤を留去後,MO-TMSi誘導体にかえてガスクロマトグラフ分析した.分析には島津製4BMPF型ガスクロマト装置を用い,分離は1%OV-1と2%QF-1カラムで行なつた.コイ尿中ステロイド(1～XIII)のMU値を標品ステロイドのそれらと対比した結果,アンドロステロン,エチオコラノロン,DHEA,アンドロステンジオール,プレグナンジオール,プレグナントリオール,コレステロール,THE,THF,コルトロンコルトロン,コルトール,β-コルトールを検出した(Table1).アンドロステロン～ コルトロンについてはさらにマススペクトル測定によつてその構造を確認し得た.
Created Date 2012.07.25
Modified Date 2022.11.11

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