Back to Results List

Export

Link to this page

Please use this URL for citations.

Search Other Services

Statistics

  • Access to this page：656 times

＜departmental bulletin paper＞
Cytoplasmic Inclusions in Rice Leaf Cells Infected with Rice Necrosis Mosaic Virus

Creator
Creator Name
長尾, 記明
Nagao, Noriaki
Affiliation
Affiliation Name
九州大学農学部植物病理学教室
Laboratory of Plant Pathology, Faculty of Agriculture, Kyushu University
Creator Name
パットポン, パトラコソン
Patpong, Patrakosol
Affiliation
Affiliation Name
九州大学農学部植物病理学教室
Laboratory of Plant Pathology, Faculty of Agriculture, Kyushu University
Creator Name
日高, 醇
Hidaka, Zyun
Affiliation
Affiliation Name
九州大学農学部植物病理学教室
Laboratory of Plant Pathology, Faculty of Agriculture, Kyushu University
Language
Japanese
Publisher
九州大學農學部
Faculty of Agriculture, Kyushu University
Date
1976-09
Source Title
Science bulletin of the Faculty of Agriculture, Kyushu University
Vol
31
Issue
1
First Page
19
Last Page
22
Publication Type
Version of Record
Access Rights
open access
JaLC DOI
https://doi.org/10.15017/23219
Related DOI
九州大學農學部學藝雜誌 || 31(1) || p19-22
Science bulletin of the Faculty of Agriculture, Kyushu University || 31(1) || p19-22
http://www.agr.kyushu-u.ac.jp/
Related URI
九州大學農學部學藝雜誌 || 31(1) || p19-22
Science bulletin of the Faculty of Agriculture, Kyushu University || 31(1) || p19-22
http://www.agr.kyushu-u.ac.jp/
Relation
九州大學農學部學藝雜誌 || 31(1) || p19-22
Science bulletin of the Faculty of Agriculture, Kyushu University || 31(1) || p19-22
http://www.agr.kyushu-u.ac.jp/

Hide fulltext details.

pdf p019 pdf 12.5 MB 475  

Details

Record ID
23219
Peer-Reviewed
Unrefereed
Subject Terms
イネえそモザイクウイルス
細胞質内封入体
膜状封入体
MI
ISSN
0368-6264
NCID
AN0005519X
Notes
Ultrathin sections of mesophyll cells of the rice(Oryza sativa L. cv. Kibiyoshi) infected with rice necrosis mosaic virus were examined with an electron microscope. Two types of cytoplasmic inclusion were observed in the cells. One was similar to the cylindrical inclusion which was observed as pinwheels or bundles mainly produced by filamentous viruses. The other was the membranous inclusion(MI) which seemed to be composed of the tubules closely assembled. Cross sections of MI showed elliptic or circular shape, about 4μm in diameter. The membranes composed of the tubules communicated with each other and associated with endoplasmic reticula at the terminal sites. The tubules were disposed in a hexagonal pattern like honeycomb and each tubule was surrounded by 6 spots which appeared like particles, about 10-12nm in diameter. The spots in cross view turned into filaments by tilting the sections at an angle of 40°. Therefore, it seems that these spots are cross view of the filaments running in parallel with the tubules but not the virus particles. MI observed in this experiment seems to have the most complex structure among the inclusions related to plant viruses reported hitherto.
イネえそモザイクウイルスに侵されたイネ葉肉細胞の電顕観察を行なった結果,健全細胞には見られない2種類の細胞質内封入体が観察された.1つはEdwardsonらが名付けた管状封入体(cylindrical inclusion)と同類と思われるもので, pinwheelやbundle型として観察される.他は管状物が密に集まってできた膜状封入体(MI)である.MIの横断像は楕円形あるいは円形で,径4μmに達するものもあった.その構造は管状物の切断される角度によって,ハチの巣状,網目状,あるいはラメラ構造を呈し,非常に複雑であった.ハチの巣状に見える部分では,個々の穴の直径は20～30nmであり,相接する3個の穴の真中に径10～12nmの電子密度の高いスポットが見られた.このスポットはその径がウイルス粒子の幅(13～14nm)に近い値であったが,切片を傾斜して観察したときの像変化から,管状物を相互に結合する繊維の断面であろうと思われる.また細胞質のvesicle化が認められる細胞ではvesicleが変化したと思われるbundle型の像が観察されたが,この構造物が管状封入体やMIの形成に関係あるかどうか明らかでない.ウイルス様粒子は細胞質内にのみ存在し,散在するかあるいはbundleに付随して見られた.封入体やウイルス様粒子の見られる細胞では葉緑体の両端が細くなったり,一部突出した形のものが多かったが,核,ミトコンドリアに変化は認められなかった.
Created Date 2012.07.25
Modified Date 2022.11.11

People who viewed this item also viewed

Back to Results List