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＜departmental bulletin paper＞
On the Mechanism of Lysozyme Catalysis : I. Hydrolysis of I-Benaoyl 2-Acetamido-2-Deoxy-β-D-Glucopyranoside

Creator
Creator Name
Koga, Daizo
古賀, 代三
Affiliation
Affiliation Name
Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University
九州大学農学部農芸化学科生物化学講座
Creator Name
Yamasaki, Nobuyuki
山崎, 信行
Affiliation
Affiliation Name
Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University
九州大学農学部農芸化学科生物化学講座
Creator Name
Hayashi, Katsuya
林, 勝哉
Affiliation
Affiliation Name
Laboratory of Sericultural Chemistry, Faculty of Agriculture, Kyushu University
九州大学農学部農芸化学科蚕糸化学講座
Creator Name
Funatsu, Masaru
船津, 勝
Affiliation
Affiliation Name
Laboratory of Biochemistry, Faculty of Agriculture, Kyushu University
九州大学農学部農芸化学科生物化学講座
Language
English
Publisher
Kyushu University
九州大学農学部
Date
1973-12
Source Title
Journal of the Faculty of Agriculture, Kyushu University
Vol
18
Issue
1
First Page
1
Last Page
9
Publication Type
Version of Record
Access Rights
open access
Crossref DOI
https://doi.org/10.5109/22842
Related DOI
九州大学農学部紀要 || 18(1) || p1-9
Journal of the Faculty of Agriculture, Kyushu University || 18(1) || p1-9
http://www.agr.kyushu-u.ac.jp/
Related URI
九州大学農学部紀要 || 18(1) || p1-9
Journal of the Faculty of Agriculture, Kyushu University || 18(1) || p1-9
http://www.agr.kyushu-u.ac.jp/
Relation
九州大学農学部紀要 || 18(1) || p1-9
Journal of the Faculty of Agriculture, Kyushu University || 18(1) || p1-9
http://www.agr.kyushu-u.ac.jp/

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Details

PISSN
0023-6152
NCID
AA00697606
Record ID
22842
Peer-Reviewed
Unrefereed
Subject Terms
Catalysis
Hydrolysis
Lysozyme
ISSN
0023-6152
NCID
AA00697606
Notes
1 -O-Benzoyl 2-acetnmido-2-deoxy-β-D-glucopyranoside was synthetized as a model compound of glycosyl-enzyme intermediate which may be considered as a possible intermediate in a lysozyme catalyzed reaction, The hydrolysis of this model compound by lynte species showed a distinctive plateau region in pH-rate profile. This may indicate that the model compound would be hydrolyzed through oxazoline intermediate which is formed by the participation of 2-acetamido group of the glucopyranose ring rf substrate as a intramolecular nucleophile. The mechanism of the lysozyme catalysis was discussed on the basis of the experimental results.
Created Date 2012.07.20
Modified Date 2022.11.11

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