The effect of nitrogenous forms on immobilization and release of nitrogen following addition of glucose and mineral nitrogen to soils was studied. The amount of nitrogen immobilized increased with increasing C/N ratio independent of the forms of nitrogen added, but the nitrogen immobilized to a maximum at a given C/N ratio was a little larger in the form of ammonia than in the form of nitrate. The amount of carbon which must be added to the soil in order to immobilize one unit of nitrogen into organic form was larger in the latter than in the former. The percentage of released nitrogen to the maximum-immobilized nitrogen at a given C/N ratio was higher in the latter than in the former.