Export

Link to this page

Please use this URL for citations.

Search Other Services

Statistics

  • Access to this page：1,993 times

＜departmental bulletin paper＞
Effect of Ammonium Excess on Tomato Plants : I. Effect of Nitrogen Sources on the Activity of some Enzymes Related to Respiratory System

Creator
Creator Name
脇本, 賢三
Wakimoto, Kenzo
Affiliation
Affiliation Name
農林水産省中国農業試験場
Creator Name
山田, 芳雄
Yamada, Yoshio
Affiliation
Affiliation Name
九州大学農学部植物栄養･肥料学教室
Laboratory of Soil Fertility and Plant Nutrition, Faculty of Agriculture, Kyushu University
Language
Japanese
Publisher
九州大學農學部
Faculty of Agriculture, Kyushu University
Date
1985-03
Source Title
Science bulletin of the Faculty of Agriculture, Kyushu University
Vol
39
Issue
4
First Page
107
Last Page
113
Publication Type
Version of Record
Access Rights
open access
JaLC DOI
https://doi.org/10.15017/22169
Related DOI
九州大學農學部學藝雜誌 || 39(4) || p107-113
Science bulletin of the Faculty of Agriculture, Kyushu University || 39(4) || p107-113
http://www.agr.kyushu-u.ac.jp/
Related URI
九州大學農學部學藝雜誌 || 39(4) || p107-113
Science bulletin of the Faculty of Agriculture, Kyushu University || 39(4) || p107-113
http://www.agr.kyushu-u.ac.jp/
Relation
九州大學農學部學藝雜誌 || 39(4) || p107-113
Science bulletin of the Faculty of Agriculture, Kyushu University || 39(4) || p107-113
http://www.agr.kyushu-u.ac.jp/

Hide fulltext details.

pdf p107 pdf 343 KB 578  

Details

Record ID
22169
Peer-Reviewed
Unrefereed
Subject Terms
トマト幼植物
アンモニウム過剰
窒素給源
酵素活性
生育障害
代謝変動
光呼吸
NH_4-N
NO_3-N
ISSN
0368-6264
NCID
AN0005519X
Notes
The present experiments were carried out in order to obtain informations about the mechanism of ammonium injury to plant by investigating changes of metabolism of tomato plants under ammonium excess. Young tomato plants were used after cultured in solutions containing different amounts of NH_4-N. The affected plants were examined for their enzymes activities related to respiration and for the accumulation of NH_4-N in their tissues. Catalase activities of leaves and roots in NH_4-N fed plants were higher than that in NO_3-N fed plants. NH_4-N contents in leaves and roots of the former plants were elevated to 100 ppm. TTC-reducing activity of roots was lower in NH_4-N fed plants than in NO_3-N fed ones, suggesting a decrease of dehydrogenase activity in the roots of the former. Polyphenol oxidase activities of leaves and roots were higher in NH_4-N fed plants than in NO_3-N fed plants, suggesting that the root browning due to lignification observed under ammonium excess was presumably related to the increase of that enzyme activity.
1.NO_3-Nを含む培養液でトマト幼植物を培養すると,根および葉身のNH_4-N含量は20-50ppmの範囲で経時的に一定しており,またNO_3-N濃度を高めてもほぼ一定のレベルで維持された.一方,NH_4-Nを含む培養液で培養するとNH_4-N含量は経時的に増加した.根では条件によっては150ppmまで上昇し,それ以上にはならなかったが,葉身では300ppm近くまで上昇した.これはNH_4-Nの集積容量が葉で高いことを示唆している.2.根と葉身,とくに葉身においてカタラーゼ活性はNO_3-N培養植物に比べNH_4-N培養植物で高かった.NH_4-N培養による葉におけるカタラーゼ活性の増大は光呼吸の増大と深い関連があるのではないかと推察した.3.根のTTC還元力はNO_3-N培養植物に比べNH_4-N培養植物で低かった.このことはエネルギー生成系の不活性を意味し,ひいてはアンモニウム過剰下での根の養水分吸収能の低下につながるものと推察した.4.根,葉身共にポリフェノールオキシダーゼ活性はNO_3-N培養植物に比べNH_4-N培養植物で高かった.根は葉身に比べ10倍以上の活性を示し,根における本酵素の役割が葉身に比べて大であると推察した.
Created Date 2012.06.29
Modified Date 2022.11.11

People who viewed this item also viewed