In order to elucidate the mechanism of reaction between autoxidized methyl linolenate (oxd MLn) and trypsin, the authors studied the functional groups participating in the reaction. The results obtained are summarized as follows. 1. Trypsin which was allowed to react with oxd MLn produced the fluorescent chromophores, which showed the excitation maximum at 340 nm and the emission maximum at 420 nm. 2. The prolongation of the autoxidation time of MLn was accompanied by the gradual increase in the florescence intensity. 3. The fluorescence was observed remarkably by the reaction with the polymer fractionated from oxd MLn, but also observed with the other fractions. 4. Trypsin was scarcely inhibited by the polymer whose carbonyl groups had been modified. In addition, acetyltrypsin, Which had been prepared by the modification of ε-amino groups in trypsin, was not inhibited by intact polymer. 5. Lys was markedly damaged in trypsin by the reaction with the polymer, compared with other amino acids. From these results, the authors concluded that the inhibition of trypsin by oxd MLn was based on the covalent binding between carbonyl groups of the polymer and ε-amino groups in trypsin.
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