Laboratory of Silkworm Sciences, Division of Genetics and Plant Breeding, Department of Applied Genetics and Pest Management, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
九州大学大学院生物資源環境科学府蚕学研究室
Laboratory of Silkworm Sciences, Division of Genetics and Plant Breeding, Department of Applied Genetics and Pest Management, Faculty of Agriculture, Kyushu University
九州大学農学研究院資源生物科学部門農業生物資源学蚕学研究室
Laboratory of Silkworm Sciences, Division of Genetics and Plant Breeding, Department of Applied Genetics and Pest Management, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University : Research Fellow of the Japan Society for the Promotion of Science
九州大学大学院生物資源環境科学府蚕学研究室 : 日本学術振興会特別研究員
Laboratory of Silkworm Sciences, Division of Genetics and Plant Breeding, Department of Applied Genetics and Pest Management, Faculty of Agriculture, Kyushu University
九州大学農学研究院資源生物科学部門農業生物資源学蚕学研究室
Laboratory of Silkworm Sciences, Division of Genetics and Plant Breeding, Department of Applied Genetics and Pest Management, Faculty of Agriculture, Kyushu University
九州大学農学研究院資源生物科学部門農業生物資源学蚕学研究室
The p53R2 is a homologue of the small subunit of mammalian ribonucleotide reductase. In human, the transcription of p53R2 is induced clearly by active form of p53 and required for DNA synthesis during cell division and DNA repair. In this study, we have isolated and determined cDNA sequence for Bombyx mori p53R2–like (p53R2–L) protein. The Bmp53R2–like protein had high homology to the Hsp53R2, but had histidine residue at a position corresponding to Y241 of Hsp53R2, which is a key residue in discriminating human p53R2 and R2. Bmp53R2–L expressed strongly in the silk grand, gonad and blood cell, in which cell division occurs actively and DNA synthesis is required. The knockdown of the Bmp53R2–L in BmN4–SID1 led to a substantial arrest in G1/S. This G1/S arrest may be caused by the retardation of DNA synthesis due to the depletion of dNTPs in nucleus under the conditions of the knockdown of Bmp53R2–L. Thus, Bmp53R2–L is a functional homolog of human R2 rather than p53R2, and considered to be indispensable for DNA synthesis and cell cycle progression in a DNA damage independent manner.
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