In this paper the authors described the result of their study on the genetic aspect of the seasonal variation of plasma protein bound iodine level, together with body temperature and respiration rate, in daughter-dam pairs of dairy cows. The pooled mean of plasma protein bound iodine levels was higher in the winter and lower in the summer, and the difference was significant at 1 % level. Highly significant daughter-dam correlation was estimated for the seasonal difference in plasma protein bound iodine levels, and the correlation coefficient was larger than both of the coefficients estimated for the seasonal differences in body temperatures and respiration rates. Highly significant correlation was also estimated between the seasonal difference in plasma protein bound iodine levels and that in body temperatures. The repeatability estimate for plasma protein bound iodine levels from July to December was relatively low. This may be related to the significant seasonal difference already described.