The results of the experiment concerning the nutritional requirement of Xanthomonas oryzae on various nitrogen compounds were described. 1. The bacterium failed to grow in Starr's basal medium, while vigorous growth was obtained when vitamin free casein hydrolysate was added to the basal medium. 2. Among twelve inorganic nitrogen compounds, fairely good growth occurred in the basal medium with ammonium oxalate as the nitrogen source, while, there was no growth with the other inorganic nitrogen compounds. 3. Among fifteen amino acids examined, vigorous growth occurred with the following amino acids: glutamic acid, aspartic acid, methionine, cystine and asparagine, but not with the other amino acids: proline, lysine, arginine, ,9-alanine, kreatin, norvaline, tyrosine, leusine, threonine and glycine. 4 . More vigorous growth occurred in culture with four amino acid mixtures than with a single addition of each of the amino acids : glutamic acid, aspartic acid, methionine and cystine.
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