On the Desoxyribonuclease activity of a Bacillus subtilis strain producing amylase - Collections

＜departmental bulletin paper＞
On the Desoxyribonuclease activity of a Bacillus subtilis strain producing amylase

Creator	Author PID K004936 Creator Name 渡辺, 健治 Watanabe, Kenji Affiliation Affiliation Name 九州大学農学部 Faculty of Agriculture, Kyushu University
Language	Japanese
Publisher	九州大學農學部
Publisher	Faculty of Agriculture, Kyushu University
Date	1959-12
Source Title	Science bulletin of the Faculty of Agriculture, Kyushu University
Vol	17
Issue	3
First Page	269
Last Page	275
Publication Type	Version of Record
Access Rights	open access
JaLC DOI	https://doi.org/10.15017/21498
Related DOI	九州大學農學部學藝雜誌 \|\| 17(3) \|\| p269-275
	Science bulletin of the Faculty of Agriculture, Kyushu University \|\| 17(3) \|\| p269-275
	http://www.agr.kyushu-u.ac.jp/
Related URI	九州大學農學部學藝雜誌 \|\| 17(3) \|\| p269-275
	Science bulletin of the Faculty of Agriculture, Kyushu University \|\| 17(3) \|\| p269-275
	http://www.agr.kyushu-u.ac.jp/
Relation	九州大學農學部學藝雜誌 \|\| 17(3) \|\| p269-275
	Science bulletin of the Faculty of Agriculture, Kyushu University \|\| 17(3) \|\| p269-275
	http://www.agr.kyushu-u.ac.jp/

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Record ID	21498
Peer-Reviewed	Unrefereed
Subject Terms	核酸分解酵素
	Desoxyribonuclease
	Dnase
	ファージ
	アミラーゼ生産性枯草病
	デオキシリボ核酸
	分解酵素
ISSN	0368-6264
NCID	AN0005519X
Notes	The existence of a DNase in the supernatant fluid of sonorated Bacillus subtilis strain was confirmed by the viscosimetric determination. The reaction is roughly of the first order and the value of the velocity constant, K, was used to compare the enzyme activity. The optimum pH of this DNase is 8.5. The enzyme is activated by both magnesium and manganese ions. The enzyme is completely destroyed by heating at 70℃ for 5 minutes.
Notes	核酸分解酵素の一つであるDesoxyribonuclease(DNase)の研究の歴史は浅く,酵素の特異性や作用機序に就いては現在でも未知の問題が多く残されている. 例えばDNaseを,膵臓等に存在し中性附近に最適pHを有するDNaseⅠと,胸腺等に広く存在し最適pHを酸性側に有するDNaseⅡに大別する事が広く行われているが,此の区別も厳密なものではない.細菌のDNaseは一般にMg^++で活性化され,pH7.0～7.5に最適値をもち,DNaseⅠに属するものと考えられている.著者は生物体内の核酸代謝,特にバイラスの誘発時や形質転換現象に於ける本酵素の挙動追求を目的として研究しつつあるが,本報では先にphage感染菌として使用したBacillus subtilis var.amlyloliquefaciens Fukumoto-K 49を材料として,其の無細胞菌体抽出液中に酵素活性を検索し得たので,其の二三の性質に就いて報告する.
Created Date	2012.03.21
Modified Date	2023.12.01

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＜departmental bulletin paper＞ On the Desoxyribonuclease activity of a Bacillus subtilis strain producing amylase

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＜departmental bulletin paper＞
On the Desoxyribonuclease activity of a Bacillus subtilis strain producing amylase