オゴノリ

The carpospore liberation in Gracilaria verrucosa not accompanied with the drying was observed. The plants growing at lower level of the littoral zone or in the tide pools do not seem to emerge everytime in the neap tides, so that such materials were used for the observation without any drying. 1) The liberation from one cystocarp proceeds very slowly than the case of the half-dried material, and taking about one hour only several hundreds spores are liberated. If the materials were dried, 20 to 30 minutes would be long enough to liberate so much amount of the spores, and so far as the author observed the most amount of the spores in one minute was 35. 2) The cystocarps of the materials collected at the same time and treated in the same way were apt to begin the spore liberation almost at the same time. The material growing in exposed station has such a tendency more considerably than the one in the sheltered. 3) The time of liberation in a day was very different in each experiment, namely in some groups the liberation occurred in the early morning and another in the daytime. The regular relation between the liberation time and the tide level could not yet been ascertained. 4) From the adult female plants, spores are liberated almost at the same time in the early season. When maturation goes on, the time becomes more irregular in each cystocarp both on the same individual or on the different individuals. 5) It often happens that the cystocarps begin the liberation just after the material were transferred to the still water in laboratory, and such cases were observed especially in the later season. It seems that the transference from flowing to still water may acts as a stimulus to spore liberation as is stated by Klebs on the zoosporangia-formation in Chlorophyceae. 6) When the cystocarps were kept under still condition, they liberate spores about one time a day and the daily liberation continues over three to four days or more. The amount of liberated spores decreases gradually day by day, and on the third or on the fourth day it shows almost none. And on the later day some cystocarps shed out the nucleoli.