The nicotine content was determined in the green tobacco leaves which were situated in the different parts of the stems. In order to avoid the loss of nicotine, both originally free or set free tduring drying, the leaves to be examined were dehydrated by means of gypsum, crushed in power, and desiccated in vacuum at room temperature. For the purpose of determining nicotine, Shedd's method which is a combination of the Kissling and regular silicotungstic acid methods was used. From the results of the experiments the writer came to the conclusion bat the nicotine content increases from the lower to the higher leaves.