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| Abstract |
In this study, soluble factors secreted by colon cancer cells (CT26) are being investigated. Conditioned medium from cultured CT26 cancer cells was collected and analyzed for the presence of immunosup...pressive compound. The presence of the active compound in the samples was constantly monitored by measuring the suppression of IFN-γ expression by ELISA using splenocytes from a normal mouse. Suppressive activity in CT26 conditioned medium was shown to be mediated by a soluble factor(s) that is less than 10kDa. After fractionation with HPLC, suppression of IFN-γ expression was detected in the two different fractions. Further processing of the fractions is needed for the identification of the specific compounds that are responsible for the suppression of tumor immunity.show more
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