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＜departmental bulletin paper＞
Preparation of Anti-fragrant Monoclonal Antibodies by the Rat Lymph Node Method and Their Characterization Using Enzyme-linked Immunosorbent Assay

Creator
Creator Name
Ohashi, Shintaro
Affiliation
Affiliation Name
Laboratory of Food Analysis, Division of Food Biotechnology, Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
Author PID
L002875
Creator Name
Nagatomo, Kazutaka
Affiliation
Affiliation Name
Laboratory of Food Analysis, Division of Food Biotechnology, Department of Bioscience and Biotechnology, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
Creator Name
Miyamoto, Koichiro
Affiliation
Affiliation Name
Program of Food Science and Technology, Course of Applied Biological Science, Department of Bioresource and Bioenvironment, School of Agriculture, Kyushu University
Author PID
K000252
Creator Name
Toko, Kiyoshi
都甲, 潔
Affiliation
Affiliation Name
Graduate School of Information Science and Electrical Engineering, Kyushu University
九州大学大学院システム情報科学研究院
Author PID
K001214
Creator Name
Miura, Norio
三浦, 則雄
Affiliation
Affiliation Name
Art, Science and Technology Center for Cooperative Research, Kyushu University
九州大学産学連携センター
Author PID
K001554
Creator Name
Matsumoto, Kiyoshi
松本, 清
Affiliation
Affiliation Name
Laboratory of Food Analysis, Division of Food Biotechnology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University
九州大学大学院農学研究院生物資源環境科学府生物機能科学専攻食品バイオ工学講座食品分析学分野
Language
English
Publisher
Faculty of Agriculture, Kyushu University
九州大学大学院農学研究院
Date
2010-02-26
Source Title
Journal of the Faculty of Agriculture, Kyushu University
Vol
55
Issue
1
First Page
91
Last Page
96
Publication Type
Version of Record
Access Rights
open access
Crossref DOI
https://doi.org/10.5109/17808
Related DOI
Journal of the Faculty of Agriculture, Kyushu University || 55(1) || p91-96
九州大学大学院農学研究院紀要 || 55(1) || p91-96
http://www.agr.kyushu-u.ac.jp/
Related URI
Journal of the Faculty of Agriculture, Kyushu University || 55(1) || p91-96
九州大学大学院農学研究院紀要 || 55(1) || p91-96
http://www.agr.kyushu-u.ac.jp/
Relation
Journal of the Faculty of Agriculture, Kyushu University || 55(1) || p91-96
九州大学大学院農学研究院紀要 || 55(1) || p91-96
http://www.agr.kyushu-u.ac.jp/

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Details

PISSN
0023-6152
NCID
AA00697606
Record ID
17808
Peer-Reviewed
Unrefereed
ISSN
0023-6152
NCID
AA00697606
Notes
In the food and beverage industries there is a great demand for a sensitive and easy detection system of fragrant compounds in order to ensure product quality control and to avoid contamination. Benzaldehyde (BZ) and furfural (FF) are typical fragrant compounds for peach?flavored beverages, and methyl anthranilate (MA) is a typical flavor of grape beverages. For sensitive detection of these flavors by immunoreaction, we prepared monoclonal antibodies against BZ, FF and MA by using the rat lymph node method. The antibody for BZ had high selectivity toward BZ compared with other fragrant compounds and high affinity with an affinity constant of 1.9×106M?1. The limit of detection (LOD) of BZ by indirect competitive ELISA (ic?ELISA) was 0.6 ng mL?1. For FF, anti?FF mAb had high selectivity toward FF and high affinity constant of 2.5×104 M?1. The LOD of FF by ic?ELISA was 100 ng mL?1. For MA, anti?MA mAb had high selectivity toward MA and high affinity constant of 1.3×105M?1. The LOD of MA by ic?ELISA was 200 ng mL?1. These monoclonal antibodies were suitable for detection of very small amounts of fragrant compounds in rinsed water of beverage industries.
Created Date 2010.12.15
Modified Date 2022.11.11

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