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＜departmental bulletin paper＞
Enzymatic Remodeling of Fatty Acid Molecules by SCDase Demonstrated that Fucosyl GM1a Possessing a Polyunsaturated Fatty Acid Induces Apoptosis in HL60 Human Promyelocytic Leukemia Cells

Creator
Creator Name
Xu, Xu
Affiliation
Affiliation Name
College of Life Science, Shenzhen University
Creator Name
Goda, Hatsumi M.
Affiliation
Affiliation Name
College of Life Science, Shenzhen University
Author PID
K001103
Creator Name
Inagaki, Masanori
Affiliation
Affiliation Name
Graduate School of Pharmaceutical Sciences, Kyushu University
Author PID
K001664
Creator Name
Okino, Nozomu
沖野, 望
Affiliation
Affiliation Name
Laboratory of Marine Resource Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University
九州大学大学院農学研究院生物機能科学部門海洋生命化学講座海洋資源化学研究室
Author PID
K001615
Creator Name
Ito, Makoto
伊東, 信
Affiliation
Affiliation Name
Bio-architecture Center, Kyushu University
九州大学バイオアーキテクチャーセンター
Language
English
Publisher
Faculty of Agriculture, Kyushu University
九州大学大学院農学研究院
Date
2009-10-29
Source Title
Journal of the Faculty of Agriculture, Kyushu University
Vol
54
Issue
2
First Page
433
Last Page
437
Publication Type
Version of Record
Access Rights
open access
Crossref DOI
https://doi.org/10.5109/16126
Related DOI
Journal of the Faculty of Agriculture, Kyushu University || 54(2) || p433-437
九州大学大学院農学研究院紀要 || 54(2) || p433-437
http://www.agr.kyushu-u.ac.jp/
Related URI
Journal of the Faculty of Agriculture, Kyushu University || 54(2) || p433-437
九州大学大学院農学研究院紀要 || 54(2) || p433-437
http://www.agr.kyushu-u.ac.jp/
Relation
Journal of the Faculty of Agriculture, Kyushu University || 54(2) || p433-437
九州大学大学院農学研究院紀要 || 54(2) || p433-437
http://www.agr.kyushu-u.ac.jp/

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Details

PISSN
0023-6152
NCID
AA00697606
Record ID
16126
Peer-Reviewed
Unrefereed
ISSN
0023-6152
NCID
AA00697606
Notes
Glycosphingolipids show heterogeneity in carbohydrate as well as lipid (ceramide) moieties. However, the biological relevance of the heterogeneity of ceramide has yet to be elucidated. To assess the significance of fatty acid molecules of glycosphingolipids, enzymatic remodeling of fatty acyl chains of glycosphingolipids was performed using sphingolipid ceramide N-deacylase which catalyzes a reversible reaction in which the N-acyl linkage of ceramide is cleaved or synthesized. Using the hydrolysis and condensation reactions of the enzyme, fucosyl GM1a having a single but different fatty acid molecule were synthesized. We found that fucosyl GM1a having polyunsaturated fatty acids (PUFA-Fuc-GM1a) inhibited the proliferation of human leukemia-derived HL60 cells, whereas parental fucosyl GM1a did not. PUFA-Fuc-GM1a, but not lyso-Fuc-GM1a which lacks fatty acid molecules, was found to induce apoptosis in HL60 cells. This study clearly shows that the fatty acid moiety of glycosphingolipids could significantly affect their pharmacological activities.
Created Date 2010.01.08
Modified Date 2022.11.11

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