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＜departmental bulletin paper＞
Construction of piggyBac-based Vectors Using Visible and Drug-resistance Marker for Introducing Foreign Genes into Silkworm Cultured Cells

Creator
Author PID
K004696
Creator Name
Tatsuke, Tsuneyuki
田附, 常幸
Affiliation
Affiliation Name
Research Fellow of the Japan Society for the Promotion of Science
九州大学生物資源環境科学府生物資源開発管理学専攻遺伝育種学講座蚕学研究室
Creator Name
Hong, Sun Mee
洪, 善美
Affiliation
Affiliation Name
Laboratory of Silkworm Sciences, Division of Genetics and Plant Breeding, Department of Applied Genetics and Pest Management, Faculty of Agriculture, Kyushu University
九州大学大学院農学研究院生物資源開発管理学部門遺伝育種学講座蚕学研究室
Author PID
L002899
Creator Name
Tobata, Hiromi
戸畑, 紘実
Affiliation
Affiliation Name
Laboratory of Silkworm Sciences, Division of Genetics and Plant Breeding, Department of Applied Genetics and Pest Management, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
九州大学生物資源環境科学府生物資源開発管理学専攻遺伝育種学講座蚕学研究室
Author PID
K003779
Creator Name
Mon, Hiroaki
門, 宏明
Affiliation
Affiliation Name
Laboratory of Silkworm Sciences, Division of Genetics and Plant Breeding, Department of Applied Genetics and Pest Management, Faculty of Agriculture, Kyushu University
九州大学大学院農学研究院生物資源開発管理学部門遺伝育種学講座蚕学研究室
Author PID
K002672
Creator Name
Lee, Jae Man
李, 在萬
Affiliation
Affiliation Name
Laboratory of Silkworm Sciences, Division of Genetics and Plant Breeding, Department of Applied Genetics and Pest Management, Faculty of Agriculture, Kyushu University
九州大学大学院農学研究院生物資源開発管理学部門遺伝育種学講座蚕学研究室
Author PID
K001547
Creator Name
Kawaguchi, Yutaka
河口, 豊
Affiliation
Affiliation Name
Laboratory of Silkworm Sciences, Division of Genetics and Plant Breeding, Department of Applied Genetics and Pest Management, Faculty of Agriculture, Kyushu University
九州大学大学院農学研究院生物資源開発管理学部門遺伝育種学講座蚕学研究室
Author PID
K000322
Creator Name
Kusakabe, Takahiro
日下部, 宜宏
Affiliation
Affiliation Name
Laboratory of Silkworm Sciences, Division of Genetics and Plant Breeding, Department of Applied Genetics and Pest Management, Faculty of Agriculture, Kyushu University
九州大学大学院農学研究院生物資源開発管理学部門遺伝育種学講座蚕学研究室
Language
English
Publisher
Faculty of Agriculture, Kyushu University
九州大学大学院農学研究院
Date
2009-10-29
Source Title
Journal of the Faculty of Agriculture, Kyushu University
Vol
54
Issue
2
First Page
397
Last Page
400
Publication Type
Version of Record
Access Rights
open access
Crossref DOI
https://doi.org/10.5109/16121
Related DOI
Journal of the Faculty of Agriculture, Kyushu University || 54(2) || p397-400
九州大学大学院農学研究院紀要 || 54(2) || p397-400
http://www.agr.kyushu-u.ac.jp/
Related URI
Journal of the Faculty of Agriculture, Kyushu University || 54(2) || p397-400
九州大学大学院農学研究院紀要 || 54(2) || p397-400
http://www.agr.kyushu-u.ac.jp/
Relation
Journal of the Faculty of Agriculture, Kyushu University || 54(2) || p397-400
九州大学大学院農学研究院紀要 || 54(2) || p397-400
http://www.agr.kyushu-u.ac.jp/

Hide fulltext details.

pdf 54_2_p397 pdf 710 KB 490  

Details

PISSN
0023-6152
NCID
AA00697606
Record ID
16121
Peer-Reviewed
Unrefereed
ISSN
0023-6152
NCID
AA00697606
Notes
Transgenic organisms have been indispensable for modern genetic analysis, such as over expression or knocked-down of the genes of interest. Transposon is one of the most efficient tools for introducing foreign DNA sequences into host genome. DNA transposon, such as piggyBac, Hermes, Minos, hobo, and mariner, have been identified in insects and have been used successfully as vectors for germline tarsnformation in various insect species. piggyBac-based transformation vectors have been broadly used in generating transgenic silkworm. However, there are few studies reporting vectors for transformation of cultured B. mori cells. In this study, we constructed new piggyBac-based vectors pPigGate, which have visible and drug selectable marker, PuroDsRed or GFPZeo in cultured cells. In order to access the utility of these vectors, we introduced BmHop2 and BmMnd1, which are meiosis specific recombination proteins, into cultured B. mori cells using the pPigGate.
Created Date 2010.01.08
Modified Date 2022.11.11

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