Creator |
|
|
Language |
|
Publisher |
|
|
Date |
|
Source Title |
|
Vol |
|
Issue |
|
First Page |
|
Last Page |
|
Publication Type |
|
Access Rights |
|
Crossref DOI |
|
Rights |
|
Related DOI |
|
|
Related URI |
|
|
Relation |
|
|
Abstract |
A semi-confocal fluorescence microscope is designed and constructed aiming to observe fluorescence excitation spectrum of soluble molecules adsorbed at the air/water interface. With a conventional con...focal fluorescence microscope, the target molecules of pyrene arises some difficulties: excitation wavelength in deep ultraviolet forces low transmission performance of microscopic objectives and low wavelength-tunable performance of an excitation laser. A total internal reflection illumination with a light-guide coupled monochromated-xenon lamp is combined with the confocal fluorescence microscope. Theoretical feasibility and performance of the microscope are discussed with preliminary results obtained.show more
|