Factors Affecting to Somatic Embryogenesis and Plant Regeneration from Callus and In Vitro Ontogeny of Doritaenopsis Taisuco Ladylip - Collections

＜departmental bulletin paper＞
Factors Affecting to Somatic Embryogenesis and Plant Regeneration from Callus and In Vitro Ontogeny of Doritaenopsis Taisuco Ladylip

Creator	Creator Name Liao, Yu-Wan Affiliation Affiliation Name Department of Horticulture, National Taiwan University
	Creator Name Huang, Pung-Ling Affiliation Affiliation Name Department of Horticulture, National Taiwan University
	Creator Name Huang, Kuang-Liang Affiliation Affiliation Name Department of Horticultural Science, National Chiayi University
	Creator Name Miyajima, Ikuo 宮島, 郁夫ミヤジマ, イクオ Affiliation Affiliation Name Institute of Tropical Agriculture, Kyushu University : Associate Professor 九州大学熱帯農学研究センター : 准教授
	Creator Name Hsu, Shan-Te Affiliation Affiliation Name Department of Horticultural Science, National Chiayi University
Language	English
Publisher	Faculty of Agriculture, Kyushu University
Publisher	九州大学大学院農学研究院
Date	2015-02-27
Source Title	Journal of the Faculty of Agriculture, Kyushu University
Vol	60
Issue	1
First Page	13
Last Page	22
Publication Type	Version of Record
Access Rights	open access
Crossref DOI	https://doi.org/10.5109/1526291
Related DOI	http://www.agr.kyushu-u.ac.jp/
Related URI	http://www.agr.kyushu-u.ac.jp/
Relation	http://www.agr.kyushu-u.ac.jp/

File	FileType	Size	Views	Description
p013	pdf	4.94 MB	833

PISSN	0023-6152
NCID	AA00697606
Record ID	1526291
Peer-Reviewed	Unrefereed
Subject Terms	ammonium nitrate
	carbohydrate
	Phalaenopsis
	protocorm–like body
	somatic embryo
ISSN	0023-6152
NCID	AA00697606
Notes	The efficient plant regeneration protocols were developed for Doritaenopsis Taisuco Ladylip through callus culture and somatic embryogenesis. The embryogenic calli were cultured on half strength Murashige and Skoog (MS) medium supplemented with 0.5 mg·L^<-1> thidiazuron, 2.5 mg·L^<-1> 2,4–dichlorophenoxyacetic acid, 30 g·L^<-1> sucrose and 3 g·L^<-1> Gelrite for proliferation. Basal salts, carbohydrate of medium obviously affected the somatic embryogenesis of callus. The highest frequency of somatic embryogenesis was obtained when embryogenic calli were cultured on half strength MS medium containing 20 g·L^<-1> maltose or 10 g·L^<-1> trehalose. The protocorm–like bodies well developed into plantlets after changing the basal salts to Hyponex,and carbohydrate to sucrose. The histological processing of somatic embryogenesis from callus was observed with light microscopy, and somatic embryo was clearly origin from single cell.
Created Date	2015.10.13
Modified Date	2022.11.11