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Regeneration from stem and leaf of cis-rubber producing plant Periploca (Periploca sepium Bunge)

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Abstract 中国産ペリプロカ(Periploca sepium Bunge)の茎葉からのシュートの再分化と植物体の再生について検討した.MS培地を基本培地とし,α-ナフタレン酢酸(NAA)と6-ベンジルアミノプリン(BAP) を組み合わせることにより,茎と葉の双方からのシュートの再分化が確認された.茎からの再分化には,BAP1μM~3μMとNAAO .1μMの組み合わせで分化率が高まった.一方,葉からの再分化...には,BAP3μM~10μMとNAAO.1~1μMの組み合わせで分化率が高まった.決定された最適な再分化培地での茎と葉における分化率はそれぞれ100%,90%と高い分化率を示した.このように,ペリプロカは組織培養による再分化と植物体の再生が容易な木本つる植物であることから,今後,ポリイソプレン生合成系の解明を目的とした遺伝子改変のためのモデル植物として利用できるものと考えられた.
A method of regenerating plantlets from stem and leaf of Periploca sepium Bunge. was designed. MS medium supplemented with 1-3μM naphthaleneacetic acid (NAA) and 0.1μM benzylaminopurine (BAP) was considered highly efficient for regeneration from stem, and leaf where it was 3-10μM BAP and 0.1-1.0μM NAA. The rate of regeneration in both Periploca was very high (stem-100%, leaf-90%) in the optimum medium. Moreover the regenerated plantlets exhibited normal morphology and grew healthily. In this study, it was showed that Periploca is a useful woody plant which can be easily utilized in tissue culture (micropropagation and regeneration). Therefore Periploca will be seen as a model plant in resolving the mechanism of rubber biosynthetic pathway.
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Created Date 2009.06.16
Modified Date 2021.03.03

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