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マツタケ発生林において,シロが形成されている林床に核酸関連物質の水溶液を散布し,細根部の菌体量および子実体発生に関して検討した.核酸関連物質として木材キシロースを主糖源に培養したトルラ酵母から抽出したリボ核酸(RNA-M)と,それを酵素処理した5'一ヌクレオチドの混合物(RNA-Nt)を取り上げた.これら40gを蒸留水4,000mlに溶解させ,林地に設けた4m^2の試験区に直接散布した.細根部の菌...体量を把握する手法として,キチン,エルゴステロール,リン脂質の定量が有効であった.散布区では細根部でこれらの指標成分が増加したことから,菌体量が高まったものと思われた.散布してから1ヶ月後に子実体発生が始まり,14日間の子実体発生期間で,散布区での発生量はRNA-Mで230g(9個),RNA-Ntで210g(7個),対照区はO-60g(O-2個)であった.マツタケ発生林で核酸関連物質を散布することによりマツタケ子実体の発生促進効果を認めた.
Ribonucleic acid (RNA-M) and 5'nucleotide mixture (RNA-Nt) were added to the mycelial colony (shiro) of Tricholoma matsutake prior to fruiting stage. Forty g ribonucleic acid derivatives was dissolved in 4,000ml distilled water, and sprayed directly to 4m^2 surface ground of the pine forest. Glucosamine, ergosterol and phospholipid were useful indicators to determine the fungal biomass in the fine roots. These indicator components in the fine roots increased after RNA-M and RNA-Nt addition, and then revealed obviously higher levels compare with nonaddition control plots. Addition of these compounds resulted to mycelial biomass increment judging from 3 indicator responses. Fruit body formation occurred around 30 days after addition of RNA-M and RNA-Nt. Total amount of fruit body was 230g (9 fruit bodies) (RNA-M) and 210g (7 fruit bodies) (RNA-Nt) during 14 days observation period. In contrast, yield of control plots were 0-60g (0-2 fruit bodies) in the experimental area. Yield and number of T. matsutake resulted increment with the addition of nucleic acid related compounds.show more
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