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＜departmental bulletin paper＞
The Influence of Organic Matter Removal Treatments on Adsorptions of DNA on an Andosol

Creator	Author PID K002235 Creator Name 佐伯, 和利 Saeki, Kazutoshi Affiliation Affiliation Name 九州大学生物環壇調節センター Biotron Institute, Kyushu University
	Author PID L002810 Creator Name 森田, 崇嗣 Morita, Takatsugu Affiliation Affiliation Name 九州大学生物環壇調節センター Biotron Institute, Kyushu University
	Creator Name 境, 雅夫 Sakai, Masao Affiliation Affiliation Name 鹿児島大学農学部 Faculty of Agrieulture, Kagoshima University
Language	Japanese
Publisher	九州大学大学院農学研究院
Publisher	Faculty of Agriculture, Kyushu University
Date	2009-02-27
Source Title	Science bulletin of the Faculty of Agriculture, Kyushu University
Vol	64
Issue	1
First Page	31
Last Page	36
Publication Type	Version of Record
Access Rights	open access
JaLC DOI	https://doi.org/10.15017/13904

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PISSN	1347-0159
NCID	AA11577672
Record ID	13904
Peer-Reviewed	Unrefereed
Subject Terms	Chemistry
Subject Terms	化学
Notes	The influence of soil organic matter on DNA adsorptions on soils was investigated by using an andosol sample including hydrogen peroxide (H202) treated and 673 K heated soils. The organic matter content of the andosol was decreased from 8.2 to 2.2% by the H202 treatment. The treatment raised DNA adsorption per unit weight on soil particles， although the surface area of the treated soil was 50% of that in the original soil. The 673K heated treatment removed the organic matter from the sample to 0.03% and decreased the surface area to 30% of the original soil. Nevertheless, the DNA adsorption of the heated soil was significantly higher than that of the original soil. These results suggested little contribution of soil organic matter to DNA adsorption on the andosol.
Notes	土壌中有機物の黒ボク土へのDNA吸着に対する影響を，土壌に過酸化水素(H202)処理，673K加熱処理を行うことによって検証した．H202処理を行うと，土壌中の全炭素含量は8.3から2.17％に減少した．H202処理土壌の比表面積は，未処理土壌にくらべ，約2分の1に減少したにもかかわらず，未処理土壌よりもDNA吸着量が高かった．673K加熱処理で，土壌中の全炭素含量は0.03％にまで減少した．673K加熱処理土壌の比表面積は，未処理土壌よりも3分の1以上少なくなった．それにもかかわらず，ほぼ完全に黒ボク土から有機物を取り除いた673K加熱処理土壌のDNA吸着量が未処理土壌よりもはるかに高かった．これらの結果から言及できるのは，土壌有機物がDNA吸着にプラスに関与していないことである．すなわち，土壌有機物にDNA分子は吸着しないようだ．
Created Date	2009.04.22
Modified Date	2022.11.11