九州大学大学院生物資源環境科学府植物資源科学専攻土壌微生物学研究室
Laboratory of Soil Biology & Biochemistry, Department of Plant Resources, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University
九州大学大学院農学研究院植物資源科学部門植物生産科学講座土壌微生物学研究室
Laboratory of Soil Biology & Biochemistry, Division of Soil Science & Plant Production, Department of Plant Resources, Agriculture, Kyushu University
Composting of the citrus processing waste was carried out using a plant scale composter, with controlling agitation(1rpm), aeration(5m^3/min)and temperature (37-42℃).After the fermentation, the C/N ratio of compost decreased from 49.4 to 23.2 and thus concentration ratio of citric acid to nitrogen was reduced from 8.98 to 3.82.When the compost was used as fertilizer for Komatsuna, the germination inhibition was improved by composting of the citrus waste. During the composting process, proliferation of yeast was observed under a microscope, while no viable bacterial species was isolated. Six kinds of yeasts were isolated from the compost and were identified by their phenotypic (morphological, biochemical and physiological characterization) and genotypic (sequencing of 18S rDNA) characteristics. They were identified as Pishia manshurica, P. membranifaciens and Schizosaccharomyces pombe.
ミカン加工残さやミカン廃棄物の通気,撹挫及び中温での加温を行った好気発酵により,易分解有機炭素の分解が促進され,相対的に窒素が増加し,結果としてC/N比およびクエン酸/窒素比が低下した.発酵により,ミカン発酵物は,小松菜の発芽に対する毒性を軽減する事が出来たが,初期生育が抑制され,上記処理では分解されない生育阻害物質の存在が考えられた.今後,発酵条件やミカン発酵物の利用方法の検討を含めて更なる改善が必要である.ミカン廃棄物の直接好気発酵に関与する微生物として,酵母が集積されており,細菌はほとんど検出されなかった.集積された酵母の形態学的,生化学的および18S rDNA部分配列から,P. manshurica, P. membranifaciens, S. pombeであることが分かった.