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Cytochrome c molecules were covalently immobilized on a self-assembled monolayer (SAM) electrode from 11-carboxyalkanethiol. The electrode with the protein/SAM/gold interfacial structure gave well-def...ined, stable redox waves which were attributed for the direct electron transfer (ET) reaction of the proteins. The formal potential was 234±4 mV (vs SHE, n=8) and the electroactive surface coverage of the protein was estimated to be 9.3±2.3 pmol cm^<-2> (n=8) which was consistent to submonolayer coverage. Detailed analysis for the ET reaction gave a rate constant of 2.5±1.0 s^<-1> (n=8).続きを見る
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