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The quantification of glycerol from Nipa Kernel Extract (NKE) using HPLC method was undertaken and resulted 4.06% concentration using hydrolysis reaction with methanol and KOH catalyst. The pure and h...ydrolyzed NKE were monitored with Hydrophilic-Interaction Chromatography (HPLC-HILIC) using isocratic elution. The chromatograms extracted from the LC were analyzed and validated and showed good performance in terms of linearity as implicated (𝑅^2=0.9928), repeatability (%RSD range from 2.6758% 𝑎𝑛𝑑 16.6130%), intermediate precision (𝑝−𝑣𝑎𝑙𝑢𝑒 (0.00119)<𝛼), limit of detection 0.0001458% (𝑤/𝑤), limit of quantification 0.0004182% (𝑤/𝑤), and accuracy (76.7105% 𝑡𝑜 82.8505%) of hydrolyzed NKE. The method executed, determines the concentration of glycerol in pure and hydrolyzed NKE sample.続きを見る
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