Antitumor potency of fractionated ethanol lignosulfonates was investigated. On the active chemical functional group (s) of the sulfonates, discussion was made comparing with two fractionated LSA. 1. By gel-filtration of ethanol LSA, three components were obtained according to their molecular weights. Two of the higher molecular components were tested for their antitumor potencies. Higher molecular E-1 was more effective than untreated LSA, while E-2 had little activity. 2. Between UL-1 and UL-2, which were fractionated by gel-filtration of LSA, higher molecular UL-1 was not so effective as E-1. UL-2 could not hamper the proliferation of tumor cells. It does not necessarily indicate that UL-2 fraction never contains antitumor ethanol LSA. We have verified that UL-2 shows a remarkable antitumor potency by elimination of toxic substances contaminated. 3. Phenol contents of these four preparations did not correlate with their antitumor potencies. These phenolic groups were assumed to enrich the activity, because the preparations which highly contained the phenols were never inert.
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