As one of the utilization of lignin sulfonate (LSA) in pulp waste liquor, we attempted to enrich its antitumor potency, which had been confirmed by us. Nitration of LSA was supposed to be one of the means. The nitrated products were fractionated by ethanol precipitation, pH adjustment and gel filtration. Antitumor potency of these fractions were discussed in relation to their molecular weights, nitrogen contents or absorption spectra. 1. Pa-1 which was obtained by the treatment of LSA with nitric acid, and Qb which was a product of nitrating acid-treatment were both effective antitumor substances. Pa-1 was more remarkable on the potency than Qb. Some of survival animals had no ascitic fluid even after death of all tumor-bearing mice of control. 2. Pa-1 and Pb which came into the same fraction by ethanol precipitation and gel fiitration or Qa and Qb precipitated by the same pH adjustment, were different from each other in their molecular weights, nitrogen contents, absorption coefficients and spectra. 3. Fractions which showed higher nitrogen content were not necessarily potent in the activity, however, effective Pa-1 and Qa had fairly high nitrogen content. 4. It was also suggested that more effective antitumor substances might be derived from LSA by suitable chemical modifications.
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