In the previous paper, the authors reported that bacterial growth in mucus was meager but it was fairly increased either by the addition of galactose or after the hydrolysis of the mucus. It is a well-known fact that the bacterial growth in protein is promoted by adding suitable nitrogen source such as peptone and amino acids. Considering these facts, three media, namely mucus solution, mucus solution plus 0.025 % galactose and mucus solution plus 0.5 % peptone, were inoculated with the mixed putrefactive bacteria obtained from the surface of loach at the initial stage of putrefaction. The putrefying process of these media was investigated for 14 days, and the following results were obtained : 1) Initial decomposition of the mucus protein was promoted by adding peptone but delayed slightly by adding galactose, though there was no considerable difference between them in the amount of final protein decomposition. 2) The combination of mucus-polysaccharide with mucus-protein does not seem to be a suitable form for bacterial growth, about half amount of mucuspolysaccharide being utilized. 3) The formation of volatile basic nitrogen was meager in the putrefaction of mucus.
Mendeley出力
