1)The pH value of normal semen in the fowl which is of white color and is called "the white semen” by the writer, has been observed to be 7.2 in average using a potentiometer with quinhydrol electrode.2) The pH value of the transparent semen has been higher than that of the white semen, and observed to be7.9. 3) The pH value of an ejaculated semen is, therefore, influenced by the immixture rate of the transparent fluid to the semen in vas deferens, and as the rate of immixture increases more and more, the pH value of the ejaculated semen shows a shift toward more alkalinity. 4) The pH value measured by a potentiometer with quinhydrol electrode shows 0.2 or 0.4 higher than that by the test paper method. 5) The transparent semen involves a small number of sperms, granules of unknown Characters, blood cells and desquamated cells, and in a certain preparations, I have been able to see one or two suspended fragments of chalazae-like appearance in the transparent semen.