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<図書>
Circadian rhythms : methods and protocols

責任表示 edited by Ezio Rosato
シリーズ Methods in molecular biology / John M. Walker, series editor ; v. 362
データ種別 図書
出版情報 Totowa, N.J. : Humana Press , c2007
本文言語 英語
大きさ xiii, 578 p. : ill. ; 24cm
概要 Circadian timing, the ability of an organism to temporally synchronize endogenous function with the external environment, has been found in every species and in many different cell types. Aiming this ...ollection of 44 papers at those who are new to circadian biology or those may merely wish to learn new techniques, Rosato (U. of Leicester, UK) presents a compendium of laboratory methods and protocols for investigating circadian timing. These include rhythmic readouts, identification of clock genes, gene expression with RNA, gene expression with proteins, in vitro systems, and microscopy analysis. A few examples of specific topics include locomotor activity in rodents, automated video image analysis of larval zebrafish locomotor rhythms, identification of clock genes using difference gel electrophoresis, quantitative polymerase chain reaction, coimmunoprecipitation assay, manipulation of mammalian cell lines for circadian studies, and immunocytochemistry on suprachiasmatic nucleus slices. Annotation ©2007 Book News, Inc., Portland, OR (booknews.com) 続きを見る
目次 Light, photoreceptors, and circadian clocks / Russell G. Foster, Mark W. Hankins, and Stuart N. Peirson
Statistical analysis of biological rhythm data / Harold B. Dowse
Rhythmic conidiation in Neurospora crassa / Cas Kramer
Monitoring and analyzing drosophila circadian locomotor activity / Mauro A. Zordan, Clara Benna, and Gabriella Mazzotta
Automated video image analysis of larval zebrafish locomotor rhythms / Gregory M. Cahill
Locomotor activity in rodents / Gianluca Tosini
Analysis of circadian leaf movement rhythms in Arabidopsis thaliana / Kieron D. Edwards and Andrew J. Millar
Detection of rhythmic bioluminescence from luciferase reporters in cyanobacteria / Shannon R. Canales ... [et al.]
Analysis of rhythmic gene expression in adult drosophila using the firefly-luciferase reporter gene / Ralf Stanewsky
Monitoring circadian rhythms in Arabidopsis thaliana using luciferase reporter genes / Anthony Hall and Paul Brown
Specialized techniques for site-directed mutagenesis in cyanobacteria / Eugenia M. Clerico, Jayna L. Ditty, and Susan S. Golden
Novel strategies for the identification of clock genes in neurospora with insertional mutagenesis / Kruno Sveric ... [et al.]
Mutagenesis with drosophila / Patrick Emery
Mutagenesis in arabidopsis / Jodi Maple and Simon G. Moller
Yeast two-hybrid screening / Jodi Maple and Simon G. Moller
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所蔵情報



理系図2F 開架 463.9/R 71 2007
003212009009853 Global 30

書誌詳細

一般注記 Includes bibliographical references and index
著者標目 Rosato, Ezio
件 名 LCSH:Circadian rhythms
MESH:Circadian Rhythm -- genetics  全ての件名で検索
MESH:Circadian Rhythm -- physiology  全ての件名で検索
MESH:Models, Biological
分 類 LCC:QP84.6
NLM:W1
NLM:QH 527
DC22:612/.022
NDC9:463.9
書誌ID 1001413040
ISBN 9781588294173
NCID BA82120584
巻冊次 ISBN:9781588294173
登録日 2010.02.08
更新日 2017.02.18

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