Mendeley出力
＜図書＞
Oligonucleotide synthesis : methods and applications
| 責任表示 | edited by Piet Herdewijn |
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| シリーズ | Methods in molecular biology / John M. Walker, series editor ; v. 288 |
| データ種別 | 図書 |
| 出版情報 | Totowa, N.J. : Humana Press , c2005 |
| 本文言語 | 英語 |
| 大きさ | xvi, 435 p. : ill. ; 23 cm |
| 概要 | Editor Herdewijn (medicinal chemistry, Katholieke U. Leuven) and his contributors describe new techniques they have developed for oligonucleotide synthesis in technology. They describe new protocols a...d applications, methods of applying oligonucleotides in new research in biology, techniques to optimize RNA synthesis for siRNA applications, and applications using small interference RNA as an inhibitor of gene expression. These 25 papers include laboratory instructions, theoretical foundations, lists of equipment and reagents, troubleshooting tips, and references. Annotation ©2004 Book News, Inc., Portland, OR (booknews.com) 続きを見る |
| 目次 | Synthesis of DNA using a new two-step cycle / Douglas J. Dellinger ... [et al.] RNA synthesis using 2'-o-(tert-butyldimethylsilyl) protection / Brian S. Sproat RNA oligonucleotide synthesis via 5'-silyl-2'-orthoester chemistry / Stephanie A. Hartsel... [et al.] Dimethylthiarum disulfide : new sulfur transfer reagent in phosphorothioates oligonucleotide synthesis / Zhiwei Wang, Quanlai Song, and Yogesh H. Sanghvi Assay for evaluating ribonuclease H-mediated degradation of RNA-antisense oligonucleotide duplexes / Annie Galarneau ... [et al.] Di- and oligonucleotide synthesis using H-phosphonate chemistry / Jacek Stawinski and Roger Strömberg Solid-phase chemistry of circular oligonucleotides / Enrique Pedroso ... [et al.] Locked nucleic acid synthesis / Henrik M. Pfundheller ... [et al.] Synthesis of DNA mimics representing HypNa-pPNA hetero-oligomers / Vladimir A. Efimov and Oksana G. Chakhmakhcheva Base-modified oligonucleotides with increased duplex stability : pyrazolo[3,4-d]pyrimidines replacing purines / Frank Seela ... [et al.] Introduction of hypermodified nucleotides in RNA / Darell Davis and Ashok C. Bajji Chemical methods for peptide-oligonucleotide conjugate synthesis / Dmitry A. Stetsenko and Michael J. Gait Biotin-labeled oligonucleotides with extraordinarily long tethering arms / A. Michael Morocho ... [et al.] Universal labeling chemistry for nucleic acid detection on DNA-chips / Ali Laayoun, Eloy ... [et al.] Post-synthetic functionalization of triple helix-forming oligonucleotides / Alexandre S. Boutorine and Jian-Sheng Sun Fluorescence-based online detection as an analytical tool in RNA electrophoresis / Ute Scheffer and Michael Göbel Design and optimization of molecular beacon real-time polymerase chain reaction assays / Jacqueline A.M. Vet and Salvatore A.E. Marras High-thoughput production of optimized primers (fimers) for whole-genome direct sequencing / Nikolai Polushin ... [et al.] Nonenzymatic template directed RNA synthesis / Marcus Hey and Michael Göbel DNAse I footprinting of small molecule binding sites on DNA / Christian Bailly, Jérome Kluza, Christopher Martin / Christian Bailly ... [et al.] Gene targeting using peptide nucleic acid / Peter E. Nielsen Nucleic acid library construction using synthetic DNA constructs / Hani S. Zaher and Peter J. Unrau In vitro selection from combinatorial nucleic acid libraries / Andres Jäschke In vitro selection procedures for identifying DNA and RNA aptamers targeted to nucleic acids and proteins / Eric Dausse ... [et al.] Short double-stranded RNA (sirna) as inhibitor of gene expression by the interference mechanism / Jean-Rémi Bertrand, Andrei Maksimenko, and Claude Malvy続きを見る |
| 電子版へのリンク | https://hdl.handle.net/2324/6976943 |
所蔵情報
| 状態 | 巻次 | 所蔵場所 | 請求記号 | 刷年 | 文庫名称 | 資料番号 | コメント | 予約・取寄 | 複写申込 | 自動書庫 |
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医学図 3F 保存・図書 | QU 25/O 46/2005 | 2005 |
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042212004001162 |
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書誌詳細
| 内容注記 | Synthesis of DNA using a new two-step cycle / Douglas J. Dellinger ... [et al.] RNA synthesis using 2'-o-(tert-butyldimethylsilyl) protection / Brian S. Sproat RNA oligonucleotide synthesis via 5'-silyl-2'-orthoester chemistry / Stephanie A. Hartsel... [et al.] Dimethylthiarum disulfide : new sulfur transfer reagent in phosphorothioates oligonucleotide synthesis / Zhiwei Wang, Quanlai Song, and Yogesh H. Sanghvi Assay for evaluating ribonuclease H-mediated degradation of RNA-antisense oligonucleotide duplexes / Annie Galarneau ... [et al.] Di- and oligonucleotide synthesis using H-phosphonate chemistry / Jacek Stawinski and Roger Strömberg Solid-phase chemistry of circular oligonucleotides / Enrique Pedroso ... [et al.] Locked nucleic acid synthesis / Henrik M. Pfundheller ... [et al.] Synthesis of DNA mimics representing HypNa-pPNA hetero-oligomers / Vladimir A. Efimov and Oksana G. Chakhmakhcheva Base-modified oligonucleotides with increased duplex stability : pyrazolo[3,4-d]pyrimidines replacing purines / Frank Seela ... [et al.] Introduction of hypermodified nucleotides in RNA / Darell Davis and Ashok C. Bajji Chemical methods for peptide-oligonucleotide conjugate synthesis / Dmitry A. Stetsenko and Michael J. Gait Biotin-labeled oligonucleotides with extraordinarily long tethering arms / A. Michael Morocho ... [et al.] Universal labeling chemistry for nucleic acid detection on DNA-chips / Ali Laayoun, Eloy ... [et al.] Post-synthetic functionalization of triple helix-forming oligonucleotides / Alexandre S. Boutorine and Jian-Sheng Sun Fluorescence-based online detection as an analytical tool in RNA electrophoresis / Ute Scheffer and Michael Göbel Design and optimization of molecular beacon real-time polymerase chain reaction assays / Jacqueline A.M. Vet and Salvatore A.E. Marras High-thoughput production of optimized primers (fimers) for whole-genome direct sequencing / Nikolai Polushin ... [et al.] Nonenzymatic template directed RNA synthesis / Marcus Hey and Michael Göbel DNAse I footprinting of small molecule binding sites on DNA / Christian Bailly, Jérome Kluza, Christopher Martin / Christian Bailly ... [et al.] Gene targeting using peptide nucleic acid / Peter E. Nielsen Nucleic acid library construction using synthetic DNA constructs / Hani S. Zaher and Peter J. Unrau In vitro selection from combinatorial nucleic acid libraries / Andres Jäschke In vitro selection procedures for identifying DNA and RNA aptamers targeted to nucleic acids and proteins / Eric Dausse ... [et al.] Short double-stranded RNA (sirna) as inhibitor of gene expression by the interference mechanism / Jean-Rémi Bertrand, Andrei Maksimenko, and Claude Malvy |
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| 一般注記 | Includes bibliographical references and index HTTP:URL=http://www.loc.gov/catdir/toc/ecip0414/2004002114.html Information=Table of contents |
| 著者標目 | Herdewijn, Piet |
| 件 名 | LCSH:Oligonucleotides -- Synthesis -- Laboratory manuals
全ての件名で検索
MESH:Oligonucleotides -- chemical synthesis -- Laboratory Manuals 全ての件名で検索 MESH:Oligonucleotides -- chemistry -- Laboratory Manuals 全ての件名で検索 |
| 分 類 | LCC:QP625.O47 NLM:W1 NLM:QU 25 DC22:572.8/5 |
| 書誌ID | 1001161594 |
| ISBN | 1588292339 |
| NCID | BA69732247 |
| 巻冊次 | ISBN:1588292339 |
| 登録日 | 2009.09.17 |
| 更新日 | 2017.02.18 |