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Critically acclaimed for more than 25 years, the Methods in Cell Biology series provides an indispensable tool for the researcher. Each volume is carefully edited by experts to contain state-of-the-ar... reviews and step-by-step protocols. Techniques are described completely so that methods are made accessible to users.
Protein Expression in Animal Cells presents information essential to researchers and students investigating cellular function. This exceptional laboratory guide covers the selection and use of expression systems that are optimal for specific exogenous proteins. Current studies often require protein expression in particular cell types, in amounts suitable for specific detection methods, and under conditions that do not otherwise perturb the specific cellular mechanisms under investigation. Such demanding requirements imply tht there is no universal vector for all experiments. This comprehensive manual describes the structure, production, host range, and practical use of diverse viral and plasmid vectors. Protocols are written at a level geared to the beginning lab student. Because it covers many versatile expression systems, expert researchers will also find this guidebook a valuable addition to their laboratory. Key Features * Describes both well-established and novel recombinant vector systems for expression of proteins * Presents methods for efficient delivery of recombinant genes into differentiated cells, tissues, and whole animals * Covers high-level and inducible systems, plus assays for protein expression * Provides beginning and advanced investigators and students with the information they need to choose the optimal viral or plasmid system for their protein * Practical, benchtop-style presentation works in lab and in the classroom 続きを見る
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Mendeley出力
