Mendeley出力
＜図書＞
Methods in cell biology
| 責任表示 | edited by David M. Prescott |
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| データ種別 | 図書 |
| 出版情報 | New York ; Tokyo : Academic Press , 1973- |
| 本文言語 | 英語 |
| 大きさ | v. ; 24 cm |
| 概要 | On preparation, delivery, and detection of fluorescent probes. Discusses factors which determine the destination of probes and methods for delivering them to specific sites in living cells. Includes i...sues related to sample physiology (microscopic cell culture), optics (basic fluorescence microscopy), and signal detection (electric photometry and imaging, immunoelectron microscopic detection of fluorophores). Annotation copyrighted by Book News, Inc., Portland, OR This is the third volume in this series covering protein translocation within and between cells. Volumes 31 and 32, Vesicular Transport, Parts A and B, treated techniques for eukaryotic cells available as of 1989. This volume, Vectorial Transport of Proteins into and across Membranes, brings together new methods and new topics as of 1991. Highlights include use of anti-idiotype antibodies, gene fusions, transcription, crosslinking, pulse-labeling, and reconstitution. Key Features * Describes contemporary approaches for studying * Protein transport * Protein topology * Organelle biogenesis * Covers methods from pre-eminent laboratories including * In vitro and in vivo investigations * Biochemical and immunological techniques * Prokaryote, chloroplast, mitochondria, peroxisome, and rough endoplasmic reticulum membranes A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation. Key Features* Describes techniques for detection of [Ca2]]I: Ca2+ - sensitive microelectrodes* Fluorescent dyes* Luminescent proteins* Includes techniques for perturbing intracellular Ca2+* Covers detailed methodology plus problems and pitfalls of each technique* Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program* Color plates illustrate techniques such as* Confocal ratio-imaging* Use of aequorin Critically acclaimed for more than 25 years, the Methods in Cell Biology series provides an indispensable tool for the researcher. Each volume is carefully edited by experts to contain state-of-the-art reviews and step-by-step protocols. Techniques are described completely so that methods are made accessible to users. This updated second edition of the popular methods book "Video Microscopy" shows how to track dynamic changes in the structure or architecture of living cells and in reconstituted preparations using video and digital imaging microscopy. Contains 10 new chapters addressing developments over the last several years. Basic information, principles, applications, and equipment are covered in the first half of the volume and more spcialized video microscopy techniques are covered in the second half. This updated second edition of the popular methods book "Video Microscopy" shows how to track dynamic changes in the structure or architecture of living cells and in reconstituted preparations using video and digital imaging microscopy. Contains 10 new chapters addressing developments over the last several years. Basic information, principles, applications, and equipment are covered in the first half of the volume and more spcialized video microscopy techniques are covered in the second half. This is a cumulative index of Volumes 53-71 of the Methods in Cell Biology series. Critically acclaimed for more than 25 years, the series provides an indispensable tool for the researcher. Each volume is carefully edited by experts to contain state-of-the-art reviews and step-by-step protocols. Techniques are described completely so that methods are made accessible to users. 続きを見る |
子書誌情報
書誌詳細
| 一般注記 | Place of publication varies Vol. 6-10, 13-15, 20 without special title "Prepared under the auspices of the American Society for Cell Biology." Vol. 27-36: Leslie Wilson, series editor Vol. 37-: Leslie Wilson, Paul Matsudaira, series editors v. 54. Cumulative subject index, volumes 31-52 v. 72. Digital microscopy: a second editon of Video Microscopy / edited by Greenfield Sluder, David E. Wolf v. 73. Cumulative index, volumes 53-71 |
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| 著者標目 | American Society for Cell Biology Prescott, David M., 1926- Wilson, Leslie Matsudaira, Paul T. |
| 書誌ID | 1000305454 |
| ISBN | 0125641060 |
| NCID | BA00653438 |
| 巻冊次 | v. 6 ; ISBN:0125641060 v. 7 ; ISBN:0125641079 v. 8 ; ISBN:0125641087 v. 9 ; ISBN:0125641095 v. 10 ; ISBN:0125641109 v. 11 ; ISBN:0125641117 v. 12 ; ISBN:0125641125 v. 13 ; ISBN:0125641133 v. 14 ; ISBN:0125641141 v. 15 ; ISBN:012564115X v. 20 ; ISBN:0125641206 v. 21A ; ISBN:0125641214 v. 21B ; ISBN:0125641400 v. 24 ; ISBN:0125641249 v. 25 ; ISBN:0125641257 v. 26 ; ISBN:0125641265 v. 27 ; ISBN:0125641273 v. 28 ; ISBN:0125641281 v. 29 ; ISBN:012564129X v. 30 ; ISBN:0125641303 v. 31 ; ISBN:0125641338 v. 32 ; ISBN:012564132X v. 33 ; ISBN:0125641338 v. 34 ; ISBN:0125641346 v. 35 ; ISBN:0125641354 v. 36 ; ISBN:0125641362 v. 40 ; ISBN:0125641419 v. 44 ; ISBN:0122870700 v. 54 ; ISBN:0125641567 v. 72 ; ISBN:0125641699 v. 73 ; ISBN:0124802818 |
| 登録日 | 2009.09.11 |
| 更新日 | 2015.05.14 |