Mendeley出力
＜図書＞
PCR : a practical approach
| 責任表示 | edited by M.J. McPherson, P. Quirke and G.R. Taylor |
|---|---|
| シリーズ | The practical approach series |
| データ種別 | 図書 |
| 出版情報 | Oxford ; New York : IRL Press at Oxford University Press , c1991-c1995 |
| 本文言語 | 英語 |
| 大きさ | 2 v. : ill. ; 24 cm |
| 概要 | Relatively simple to perform, yet extremely diverse in its applications, polymerase chain reaction (PCR) has become an important tool in many areas of biomedical research. It enables scientists to pro...uce large quantities of DNA from minute amounts of starting material. This book provides a general introduction to PCR for researchers new to the area. It covers gene analysis at the level of restriction enzyme polymorphism, point mutation and sequence analysis. Methods for using different source materials - cloned DNA, genomic DNA, RNA, nucleic acid from archive material and PCR products themselves - are given. The emphasis throughout is on the practical applications of the technique and detailed experimental protocols are included at all stages, as well as valuable advice on avoiding pitfalls and optimizing results. Although other manuals exist, this is the most up-to-date work of its kind and has the advantage of being presented in the popular Practical Approach format. It will be of value to researchers and advanced students working in a wide variety of fields, from molecular biology to medicine. Relatively simple to perform, yet extremely diverse in its applications, polymerase chain reaction (PCR) has become an important tool in many areas of biomedical research. It enables scientists to produce large quantities of DNA from minute amounts of starting material. This book provides a general introduction to PCR for researchers new to the area. It covers gene analysis at the level of restriction enzyme polymorphism, point mutation and sequence analysis. Methods for using different source materials - cloned DNA, genomic DNA, RNA, nucleic acid from archive material and PCR products themselves - are given. The emphasis throughout is on the practical applications of the technique and detailed experimental protocols are included at all stages, as well as valuable advice on avoiding pitfalls and optimizing results. Although other manuals exist, this is the most up-to-date work of its kind and has the advantage of being presented in the popular Practical Approach format. It will be of value to researchers and advanced students working in a wide variety of fields, from molecular biology to medicine. PCR is now one of the most widely used of basic molecular biology techniques and is an indispensable research tool for the molecular biologist. The basic PCR technique provides the cornerstone for in vitro DNA amplification, upon which further features can be built to expand the range of PCR-based applications. PCR: A Practical Approach Volume 2 is not a revised version of PCR: A Practical Approach, but sets out to address some of the new and exciting applications of PCR including direct sequencing, mRNA quantitation and expression, genomic DNA mapping, and mutation analysis. 続きを見る |
所蔵情報
| 状態 | 巻次 | 所蔵場所 | 請求記号 | 刷年 | 文庫名称 | 資料番号 | コメント | 予約・取寄 | 複写申込 | 自動書庫 |
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理系図 自動書庫 | 463/Ma 23/p | 1991 |
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068342191003488 |
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書誌詳細
| 別書名 | 異なりアクセスタイトル:Polymerase chain reaction : a practical approach |
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| 一般注記 | Includes bibliographical references and index |
| 著者標目 | McPherson, M. J. (Michael J.) Taylor, G. R. (Graham R.) Quirke, P. (Philip) |
| 件 名 | LCSH:Polymerase chain reaction MESH:DNA Replication MESH:Gene Amplification |
| 分 類 | LCC:QP606.D46 NLM:QH450 DC20:574.87/3282 |
| 書誌ID | 1000123842 |
| ISBN | 019963226X |
| NCID | BA13105821 |
| 巻冊次 | [v. 1] : pbk ; ISBN:019963226X ; PRICE:£30.00 v. 1 : pbk ; ISBN:0199631964 ; PRICE:£19.50 v. 2 : hbk ; ISBN:0199634246 v. 2 : hbk ; ISBN:0199634254 |
| 登録日 | 2009.09.10 |
| 更新日 | 2009.09.10 |