Target-Cell-Specific Left–Right Asymmetry of NMDA Receptor Content in Schaffer Collateral Synapses in ε1/NR2A Knock-Out Mice

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Target-Cell-Specific Left–Right Asymmetry of NMDA Receptor Content in Schaffer Collateral Synapses in ε1/NR2A Knock-Out Mice

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Kyushu Univ. Production Kyushu Univ. Production
Responsibility:
Wu, Yue(Department of Physiology, School of Life Science, Graduate University for Advanced Studies | Division of Cerebral Structure, National Institute for Physiological Sciences)
Kawakami, Ryosuke(Department of Biology, Faculty of Science, Kyushu University)
Shinohara, Yoshiaki(Division of Cerebral Structure, National Institute for Physiological Sciences)

Fukaya, Masahiro(Department of Anatomy, Hokkaido University School of Medicine)
Sakimura, Kenji(Department of Cellular Neurobiology, Brain Research Institute, Niigata University)
Mishina, Masayoshi(Department of Molecular Neurobiology and Pharmacology, School of Medicine, University of Tokyo)
Watanabe, Masahiko
Ito, Isao(Department of Biology, Faculty of Science, Kyushu University)
Shigemoto, Ryuichi(Solution-Oriented Research for Science and Technology, Japan Science and Technology Agency | Department of Physiology, School of Life Science, Graduate University for Advanced Studies | Division of Cerebral Structure, National Institute for Physiological Sciences)
川上, 良介(九州大学大学院理学研究院生物科学部門)
篠原, 良章(自然科学研究機構・生理学研究所 大脳皮質機能研究系・脳形態解析)
深谷, 昌弘(北海道大学大学院医学研究科解剖学部門)
崎村, 建司(新潟大学脳研究所 基礎神経科学部門)
三品, 昌美
渡辺, 雅彦
伊藤, 功(九州大学大学院理学研究院生物科学部門)
重本, 隆一(自然科学研究機構・生理学研究所 大脳皮質機能研究系・脳形態解析)

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Language:
English
Publication info:
Journal of NeuroScience. 25, (40), pp. 9213-9226, 2005-10-05. Society for Neuroscience
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Abstract:
Input-dependent left–right asymmetry of NMDA receptor ε2 (NR2B) subunit allocation was discovered in hippocampal Schaffer collateral (Sch) and commissural fiber pyramidal cell synapses (Kawakami et al., 2003). To investigate whether this asymmetrical ε2 allocation is also related to the types of the postsynaptic cells, we compared postembedding immunogold labeling for ε2 in left and right Sch synapses on pyramidal cells and interneurons. To facilitate the detection of ε2 density difference, we used ε1 (NR2A) knock-out (KO) mice, which have a simplified NMDA receptor subunit composition. The labeling density for ε2 but not ε1 (NR1) and subtype 2/3 glutamate receptor (GluR2/3) in Sch-CA1 pyramidal cell synapses was significantly different between the left and right hippocampus with opposite directions in strata oriens and radiatum; the left to right ratio of ε2 labeling density was 1:1.50 in stratum oriens and 1.44:1 in stratum radiatum.Nosignificant difference, however, was detected inCA1 stratum radiatum between the left and right Sch-GluR4-positive (mostly parvalbumin-positive) and Sch-GluR4-negative interneuron synapses. Consistent with the anatomical asymmetry, the amplitude ratio of NMDA EPSCs to non-NMDA EPSCs in pyramidal cells was approximately two times larger in right than left stratum radiatum and vice versa in stratum oriens of ε1 KO mice. Moreover, the amplitude of long-term potentiation in the Sch-CA1 synapses of left stratum radiatum was significantly larger than that in the right corresponding synapses. These results indicate that the asymmetry of ε2 distribution is target cell specific, resulting in the left–right difference in NMDA receptor content and plasticity in Sch-CA1 pyramidal cell synapses in ε1 KO mice. Read more
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