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C. elegans : methods and applications
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| Table of Contents | An overview of C. elegans biology Kevin Strange Comparative genomics in C. elegans, C. briggsae, and other Caenorhabditis species Avril Coghlan, Jason E. Stajich, and Todd W. Harris WormBase: methods for data mining and comparative genomics Todd W. Harris and Lincoln D. Stein C. elegans deletion mutant screening Robert J. Barstead and Donald G. Moerman Insertional mutagenesis in C. elegans using the drosophila transposon Mos1: a methods for the rapid identification of mutated genes Jean-Louis Bessereau Single nucleotide polymorphism mapping M. Wayne Davis and Marc Hammarlund Creation of transgenic lines using microparticle bombardment methods Vida Praitis Construction of plasmids for RNA interference and in vitro transcription of double-stranded RNA Lisa Timmons Delivery methods for RNA interference in C. elegans Lisa Timmons Functional genomic approaches in C. elegans Todd Lamitina Assays for toxicity studies in C. elegans with Bt crystal proteins Larry J. Bischof, Danielle L. Huffman, and Raffi V. Aroian Fluorescent reporter methods Harald Hutter Electrophysiological analysis of neuronal and muscle function in C. elegans Michael M. Francis and Andres Villu Maricq Sperm and oocyte isolation methods for biochemical and proteomic analysis Michael A. Miller Preservation of C. elegans tissue via high-pressure freezing and freeze-substitution for ultrastructural analysis and immunocytochemistry Robby M. Weimer Intracellular pH measurements in vivo using green fluorescent protein variants Keith Nehrke Automated imaging of C. elegans behavior Christopher J. Cronin, Zhaoyang Feng, and William R. Schafer Intracellular Ca2+ imaging in C. elegans Rex A. Kerr and William R. Schafer In vitro culture of C. elegans somatic cells Kevin Strange and Rebecca Morrison Techniques for analysis, sorting, and dispensing of C. elegans on the COPAS flow-sorting system Rock Pulak.show more |
| View fulltext | Springer Nature Complete Protocols: 2006 |
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| Created Date | 2023.09.29 |
| Modified Date | 2024.01.30 |