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＜departmental bulletin paper＞
Isolation of Carp Complement Factor B and Formation of the C3-Convertase

Creator
Author PID
K001611
Creator Name
Nakao, Miki
中尾, 実樹
ナカオ, ミキ
Affiliation
Affiliation Name
Laboratory of Marine Biochemistry ,Division of Marine Biological Chemistry,Department of Bioscience and Biotechnology,Faculty of Agriculture,Kyushu University
Author PID
K003307
Creator Name
Nakahara, Makiko
中原, マキ子
ナカハラ, マキコ
Affiliation
Affiliation Name
Laboratory of Marine Biochemistry,Division of Marine Biological Chemistry,Department of Bioscience and Biotechnology,Graduate School of BIoresource and Bioenvironmental Sciences,Kyushu University
Author PID
K001531
Creator Name
Yano, Tomoki
矢野, 友紀
ヤノ, トモキ
Affiliation
Affiliation Name
Laboratory of Marine Biochemistry ,Division of Marine Biological Chemistry,Department of Bioscience and Biotechnology,Faculty of Agriculture,Kyushu University
Language
English
Publisher
Faculty of Agriculture, Kyushu University
九州大学大学院農学研究院
Date
2004-02-01
Source Title
Journal of the Faculty of Agriculture, Kyushu University
Vol
49
Issue
1
First Page
101
Last Page
110
Publication Type
Version of Record
Access Rights
open access
Crossref DOI
https://doi.org/10.5109/4570
Related DOI
Journal of the Faculty of Agriculture, Kyushu University || 49(1) || p101-110
九州大学大学院農学研究院紀要 || 49(1) || p101-110
http://www.agr.kyushu-u.ac.jp/index.html
Related URI
Journal of the Faculty of Agriculture, Kyushu University || 49(1) || p101-110
九州大学大学院農学研究院紀要 || 49(1) || p101-110
http://www.agr.kyushu-u.ac.jp/index.html
Relation
Journal of the Faculty of Agriculture, Kyushu University || 49(1) || p101-110
九州大学大学院農学研究院紀要 || 49(1) || p101-110
http://www.agr.kyushu-u.ac.jp/index.html

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Details

PISSN
0023-6152
NCID
AA00697606
Record ID
4570
Peer-Reviewed
Unrefereed
ISSN
0023-6152
NCID
AA00697606
Notes
Complement factor B (Bf) is a keycomponent that forms a catalytic side of C3-convertase of the alternative activation pathway. However, the functional assay and isolation method has been developed poorly for Bf in fish. We describe here a hemolytic assay method for Bf of carp (Cyprinus carpio). The assay derised from prified carp C3 and factor D (Df) is based on the action of Bf which cleaves C3 in the presence of Df. Using this assay method as a probe, carp Bf was isolated from serum, and proved to be a protein comopsed of a single polypeptide with a molecular mass of 93 kDa. This C3-convertase of tje alternative pathway of carp completement was assembled from purified C3, Bf and generation of C3b, the initial biologically active fragment derived from C3, was confirmed, suggesting a well conserved mechanism of the formation of the alternative pathway C3-convertase.
Created Date 2009.04.22
Modified Date 2022.11.11

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