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Cell cycle checkpoints : methods and protocols
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| 目次 | 1. Studying G2 DNA damage checkpoints using the fission yeast schizosaccharomyces pombe Nicholas Willis and Nicholas Rhind 2. Studying S-phase DNA damage checkpoints using the fission yeast schizosaccharomyces pombe Nicholas Willis and Nicholas Rhind 3. Methods for studying the G2 DNA damage checkpoint in mammalian cells Claudia Tapia-Alveal and Matthew J. O'Connell 4. Evaluating spindle assembly checkpoint competence in mouse oocytes using immunoblotting Hayden Homer 5. Analysis of changes in protein level and subcellular localization during cell cycle progression using the budding yeast saccharomyces cerevisiae Xiaorong Wu, Lili Liu, and Mingxia Huang 6. Studying cell cycle checkpoints using drosophila cultured cells Katarzyna Siudeja, Jannie de Jong, and Ody C.M. Sibon 7. In vivo live-analysis of cell cycle checkpoints in drosophila early embryos Saeko Takada and Byeong J. Cha 8. Using drosophila larval imaginal discs to study low-dose radiation-induced cell cycle arrest Shian-Jang Yan and Willis X. Li 9.Screening for radiation sensitizers of drosophila checkpoint mutants Mara Gladstone and Tin Tin Su 10. Study of cell cycle checkpoints using xenopus cell-free extracts Seetha V. Srinivasan and Jean Gautier 11. Analyzing DNA replication dynamics of genotoxin-treated cells using velocity sedimentation Tovah A. Day, Chris Sproul, Marila Cordeiro-Stone, and Cyrus Vaziri 12. Methods for studying checkpoint kinases-chk1 Claudia Tapia-Alveal and Matthew J. O'Connell 13. A human cell extract-based assay for the activation of ATM and ATR checkpoint kinases Bunsyo Shiotani and Lee Zou 14. Analyzing p53 regulated DNA damage checkpoints by flow cytometry Lois Resnick-Silverman and James J. Manfredi 15. Using drosophila S2 cells to measure S phase-coupled protein destruction via flow cytometry Jean M. Davidson and Robert J. Duronio 16. Assessing G₁-to-S-phase progression after genotoxic stress Michael DeRan, Mary Pulvino, and Jiyong Zhao 17. Indirect immunofluorescence for monitoring spindle assembly and disassembly in yeast Jacob W.P. Keeling and Rita K. Miller 18. Detecting recruitment of DNA damage response factors through the eChIP approach Yucai Wang and Lei Li 19. Methods to study cancer therapeutic drugs that target cell cycle checkpoints Yun Dai and Steven Grant.続きを見る |
| 本文を見る | Springer Nature Complete Protocols: 2011 |
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| 登録日 | 2023.09.29 |
| 更新日 | 2024.01.30 |