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Mitosis : methods and protocols
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| 目次 | Knock-in and knock-out: the use of reverse genetics in somatic cells to dissect mitotic pathways A.R. Barr, D. Zyss and F. Gergely Functional dissection of mitotic regulators through gene targeting in human somatic cells E. Berdougo, M.E. Terret and P.V. Jallepalli RNAi in Drosophila S2 cells as a tool for studying cell cycle progression M. Bettencourt-Dias and G. Goshima Production of mitotic regulators using an autoselection system for protein expression in budding yeast M. Geymonat, A. Spanos and S. Sedgwick Hydrodynamic analysis of human kinetochore complexes during mitosis S.E. McClelland and A.D. McAinsh Isolation of protein complexes involved in mitosis and cytokinesis from Drosophila cultured cells P.P. D'Avino ... [et al.] Automated live microscopy to study mitotic gene function in fluorescent reporter cell lines M.H. Schmitz and D.W. Gerlich Electron tomography of microtubule end-morphologies in C. elegans embryos E. O'Toole and T. Muller-Reichert Dissecting mitosis with laser microsurgery and RNAi in Drosophila cells A.J. Pereira ... [et al.] Fluorescence imaging of the centrosome cycle in mammalian cells S.L. Prosser and A.M. Fry Visualization of fluorescence-tagged proteins in fission yeast: the analysis of mitotic spindle dynamics using GFP-tubulin under the native promoter M. Sato, M. Toya and T. Toda Analysing kinetochore function in human cells: spindle checkpoint and chromosome congression C. Klebig ... [et al.] Probing kinetochore structure and function using Xenopus laevis frog egg extracts M.J. Emanuele and P.T. Stukenberg Live cell imaging of kinetochore capture by microtubules in budding yeast K. Tanaka and T.U. Tanaka The spindle checkpoint: assays for the analysis of spindle checkpoint arrest and recovery J. Fernius and K.G. Hardwick Measuring proteolysis in mitosis C. Lindon and B.D. Fiore An in vitro assay for Cdc20-dependent mitotic anaphase-promoting complex activity from budding yeast S.C. Schuyler and A.W. Murray In vitro assays for the anaphase-promoting complex/cyclosome (APC/C) in Xenopus egg extracts H. Yamano ... [et al.] Preparation of synchronized human cell extracts to study ubiquitination and degradation A. Williamson, L. Jin and M. Rape Biochemical analysis of the anaphase promoting complex: activities of E2 enzymes and substrate competitive (pseudosubstrate) inhibitors M.K. Summers and P.K. Jackson.続きを見る |
| 本文を見る | Springer Nature Complete Protocols: 2009 |
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| 登録日 | 2023.09.29 |
| 更新日 | 2024.01.30 |