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Microbial gene essentiality : protocols and bioinformatics
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目次 | Overview of whole-genome essentiality analysis Karen Joy Shaw Pt. I. Experimental protocols. IA. Populational genome-wide essentiality screens. Transposon-based strategies for the identification of essential bacterial genes William S. Reznikoff and Kelly M. Winterberg Identification and analysis of essential genes in Haemophilus influenzae Sandy M.S. Wong and Brian J. Akerley Transposon site hybridization in Mycobacterium tuberculosis Jeffrey P. Murry ... [et al.] Essential genes in the infection model of Pseudomonas aeruginosa PCR-based signature-tagged mutagenesis François Sanschagrin, Irena Kukavica-Ibrulj, and Roger C. Levesque Whole-genome detection of conditionally essential and dispensable genes in Escherichia coli via genetic footprinting Michael D. Scholle and Svetlana Y. Gerdes Generating a collection of insertion mutations in the Staphylococcus aureus genome using bursa aurealis Taeok Bae ... [et al.] Multipurpose transposon insertion libraries for large-scale analysis of gene function in yeast Anuj Kumar IB. Systematic collections of knockout mutants. How to make a defined near-saturation mutant library. Case 1: Pseudomonas aeruginosa PAO1 Michael A. Jacobs Comparing insertion libraries in two Pseudomonas aeruginosa strains to assess gene essentiality Nicole T. Liberati ... [et al.] The construction of systematic in-frame, single-gene knockout mutant collection in Escherichia coli K-12 Tomoya Baba and Hirotada Mori The applications of systematic in-frame, single-gene knockout mutant collection of Escherichia coli K-12 Tomoya Baba ... [et al.] A novel, simple, high-throughput method for isolation of genome-wide transposon insertion mutants of Escherichia coli K-12 Takeyoshi Miki, Yoshihiro Yamamoto, and Hideo Matsuda High-throughput creation of a whole-genome collection of yeast knockout strains Angela M. Chu and Ronald W. Davis Analysis of genetic interactions on a genome-wide scale in budding yeast: diploid-based synthetic lethality analysis by microarray Pamela B. Meluh ... [et al.] IC. Genome minimization. Scarless engineering of the Escherichia coli genome Tamás Fehér ... [et al.] Minimization of the Escherichia coli genome using the Tn5-targeted Cre/loxP excision system Byung Jo Yu and Sun Chang Kim Construction of long chromosomal deletion mutants of Escherichia coli and minimization of the genome Jun-ichi Kato and Masayuki Hashimoto ID. Conditional knockouts. Identification of essential genes in Staphylococcus aureus by construction and screening of conditional mutant library Dezhong Yin and Yinduo Ji Techniques for the isolation and use of conditionally expressed antisense RNA to achieve essential gene knockdowns in Staphylococcus aureus Allyn Forsyth and Liangsu Wang Introduction of conditional lethal amber mutations in Escherichia coli Christopher D. Herring Pt. II. Bioinformatics. IIA. Statistics. Statistical methods for building random transposon mutagenesis libraries Oliver Will Statistical evaluation of genetic footprinting data Gábor Balázsi Modeling competitive outgrowth of mutant populations : why do essentiality screens yield divergent results? Alexander I. Grenov and Svetlana Y. Gerdes Statistical analysis of fitness data determined by TAG hybridization on microarrays Brian D. Peyser, Rafael Irizarry, and Forrest A. Spencer IIB. Data integration and modeling. Profiling of Escherichia coli chromosome database Yukiko Yamazaki, Hironori Niki, and Jun-ichi Kato Gene essentiality analysis based on DEG, a database of essential genes Chun-Ting Zhang and Ren Zhang Detection of essential genes in Streptococcus pneumoniae using bioinformatics and allelic replacement mutagenesis Jae-Hoon Song and Kwan Soo Ko Design and application of genome-scale reconstructed metabolic models Isabel Rocha, Jochen Förster, and Jens Nielsen Predicting gene essentiality using genome-scale in silico models Andrew R. Joyce and Bernhard Ø. Palsson Comparative approach to analysis of gene essentiality Andrei L. Osterman and Svetlana Y. Gerdes.続きを見る |
本文を見る | Springer Nature Complete Protocols: 2008 |
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登録日 | 2023.09.29 |
更新日 | 2024.01.30 |