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Microbial gene essentiality : protocols and bioinformatics

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目次 Overview of whole-genome essentiality analysis
Karen Joy Shaw
Pt. I. Experimental protocols. IA. Populational genome-wide essentiality screens. Transposon-based strategies for the identification of essential bacterial genes
William S. Reznikoff and Kelly M. Winterberg
Identification and analysis of essential genes in Haemophilus influenzae
Sandy M.S. Wong and Brian J. Akerley
Transposon site hybridization in Mycobacterium tuberculosis
Jeffrey P. Murry ... [et al.]
Essential genes in the infection model of Pseudomonas aeruginosa PCR-based signature-tagged mutagenesis
François Sanschagrin, Irena Kukavica-Ibrulj, and Roger C. Levesque
Whole-genome detection of conditionally essential and dispensable genes in Escherichia coli via genetic footprinting
Michael D. Scholle and Svetlana Y. Gerdes
Generating a collection of insertion mutations in the Staphylococcus aureus genome using bursa aurealis
Taeok Bae ... [et al.]
Multipurpose transposon insertion libraries for large-scale analysis of gene function in yeast
Anuj Kumar
IB. Systematic collections of knockout mutants. How to make a defined near-saturation mutant library. Case 1: Pseudomonas aeruginosa PAO1
Michael A. Jacobs
Comparing insertion libraries in two Pseudomonas aeruginosa strains to assess gene essentiality
Nicole T. Liberati ... [et al.]
The construction of systematic in-frame, single-gene knockout mutant collection in Escherichia coli K-12
Tomoya Baba and Hirotada Mori
The applications of systematic in-frame, single-gene knockout mutant collection of Escherichia coli K-12
Tomoya Baba ... [et al.]
A novel, simple, high-throughput method for isolation of genome-wide transposon insertion mutants of Escherichia coli K-12
Takeyoshi Miki, Yoshihiro Yamamoto, and Hideo Matsuda
High-throughput creation of a whole-genome collection of yeast knockout strains
Angela M. Chu and Ronald W. Davis
Analysis of genetic interactions on a genome-wide scale in budding yeast: diploid-based synthetic lethality analysis by microarray
Pamela B. Meluh ... [et al.]
IC. Genome minimization. Scarless engineering of the Escherichia coli genome
Tamás Fehér ... [et al.]
Minimization of the Escherichia coli genome using the Tn5-targeted Cre/loxP excision system
Byung Jo Yu and Sun Chang Kim
Construction of long chromosomal deletion mutants of Escherichia coli and minimization of the genome
Jun-ichi Kato and Masayuki Hashimoto
ID. Conditional knockouts. Identification of essential genes in Staphylococcus aureus by construction and screening of conditional mutant library
Dezhong Yin and Yinduo Ji
Techniques for the isolation and use of conditionally expressed antisense RNA to achieve essential gene knockdowns in Staphylococcus aureus
Allyn Forsyth and Liangsu Wang
Introduction of conditional lethal amber mutations in Escherichia coli
Christopher D. Herring
Pt. II. Bioinformatics. IIA. Statistics. Statistical methods for building random transposon mutagenesis libraries
Oliver Will
Statistical evaluation of genetic footprinting data
Gábor Balázsi
Modeling competitive outgrowth of mutant populations : why do essentiality screens yield divergent results?
Alexander I. Grenov and Svetlana Y. Gerdes
Statistical analysis of fitness data determined by TAG hybridization on microarrays
Brian D. Peyser, Rafael Irizarry, and Forrest A. Spencer
IIB. Data integration and modeling. Profiling of Escherichia coli chromosome database
Yukiko Yamazaki, Hironori Niki, and Jun-ichi Kato
Gene essentiality analysis based on DEG, a database of essential genes
Chun-Ting Zhang and Ren Zhang
Detection of essential genes in Streptococcus pneumoniae using bioinformatics and allelic replacement mutagenesis
Jae-Hoon Song and Kwan Soo Ko
Design and application of genome-scale reconstructed metabolic models
Isabel Rocha, Jochen Förster, and Jens Nielsen
Predicting gene essentiality using genome-scale in silico models
Andrew R. Joyce and Bernhard Ø. Palsson
Comparative approach to analysis of gene essentiality
Andrei L. Osterman and Svetlana Y. Gerdes.
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本文を見る Springer Nature Complete Protocols: 2008

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登録日 2023.09.29
更新日 2024.01.30